2,4-D Hyper Accumulation Induced Cellular Responses of Azolla pinnata R. Br. to Sustain Herbicidal Stress  

作　　者：Arnab Kumar De Arijit Ghosh Debabrata Dolui Indraneel Saha Malay Kumar Adak 

机构地区：[1]Plant Physiology and Plant Molecular Biology Research Unit,Department of Botany,University of Kalyani,West Bengal,India

出　　处：《Phyton-International Journal of Experimental Botany》2020年第4期999-1017,共19页国际实验植物学杂志（英文）

基　　金：This work isfinancially supported by DST-PURSE II program,DST,Govt.of INDIA on University of Kalyani.

摘　　要：In the present experiment with ongoing concentration(0µM,100µM,250µM,500µM and 1000µM)of 2,4-D,the responses of Azolla pinnata R.Br.was evaluated based on cellular functions.Initially,plants were significantly tolerated up to 1000µM of 2,4-D with its survival.This was accompanied by a steady decline of indole acetic acid(IAA)concentration in tissues with 78.8%over the control.Membrane bound H^(+)-ATPase activity was over expressed within a range of 1.14 to 1.25 folds with activator(KCl)and decreased within a range of 57.3 to 74.6%in response to inhibitor(Vanadate)application.With regards to IAA metabolism,plants recorded a linear increase with wall bound oxidase activity up to maximum concentration of 2,4-D.The variations were more moderated when wall bound IAA-oxidase recorded a linear increase proportionate to the 2,4-D concentrations.This was more extended with the presence of different isoforms of IAA-oxidase which was much more pronounced with distinct polymorphisms of expressed proteins,however,not independent to the 2,4-D concentrations.Polyamines like spermine,spermidine and putrescine(spm,spd and put)were not consistent in concentration with the dosages of 2,4-D.Besides these,plants were induced to apoplastic NAD(P)H oxidase activity maximally by 1.6 folds under 500µM 2,4-D over control.Still,putrescine responded more or less consistently and recorded maximally 11.9 folds at 500µM 2,4-D as compared to the control.NAD(P)H oxidase activity recorded maximally 1.6 folds against control and remain consistent throughout the concentrations of 2,4-D.GPX along with APX were more linear in responses through the concentration of 2,4-D except CAT as compared to control.On enzymatic antioxidative activity,peroxidases(GPX and APX)were overexpresed in a similar manner except for catalase with a non-significant rise.In stabilization of cellular redox,glutathione reductase attended maximum value by 2.45 folds at 1000µM evidenced with significant variations in protein polymorphism.The sensitivity of 2,4-D also ap

关 键 词：AZOLLA 2 4-D antioxidative enzymes comet assay HPLC 

分 类 号：O62[理学—有机化学]