循环miR-135a水平与HDL抗氧化功能的关联性  

Correlation between circulating miR-135a level and antioxidant function of HDL

作　　者：葛海静 吕欣桐 何涛 喻红[2] 鲁志兵 杜芬[2] GE Haijing;L Xintong;HE Tao;YU Hong;LU Zhibing;DU Fen(Department of Cardiology,Zhongnan Hospital of Wuhan University&Institute of Myocardial Injury and Repair of Wuhan University,Wuhan,Hubei 430071,China;School of Basic Medical Sciences,Wuhan University&Hubei Provincial Key Laboratory of Developmentally Originated Disease,Wuhan,Hubei 430071,China)

机构地区：[1]武汉大学中南医院心血管内科武汉大学心肌损伤与修复研究所,湖北省武汉市430071 [2]武汉大学基础医学院湖北省发育源性疾病重点实验室,湖北省武汉市430071

出　　处：《中国动脉硬化杂志》2022年第3期198-204,共7页Chinese Journal of Arteriosclerosis

基　　金：国家自然科学基金面上项目(81670412、81670423);武汉大学中南医院转化医学及交叉研究联合基金项目(ZNLH201907)。

摘　　要：目的研究血浆高密度脂蛋白(HDL)功能与循环微小RNA(miRNA)水平的相关性。方法利用失功能HDL模型小鼠清道夫受体B族Ⅰ型缺陷(SR-BⅠ^(-/-))小鼠发掘特异性miRNA谱的变化;收集30例2020年9月—2021年5月就诊于武汉大学中南医院心血管内科的冠状动脉粥样硬化性心脏病(CAD)患者血浆并检测miRNA的变化,对照组为同时期住院的非CAD患者,两组血浆高密度脂蛋白胆固醇(HDLC)水平均高于1.04 mmol/L。结果与野生型小鼠相比,SR-BⅠ^(-/-)小鼠血浆miR-135a水平显著减少。与对照组相比,CAD组血浆对氧磷酶1(PON-1)活性降低;对照组PON-1活性与HDLC水平正相关,但CAD组PON-1活性与HDLC水平无相关性。CAD组血浆miR-135a水平降低,且与PON-1活性正相关。CAD组血浆miR-17显著降低,miR-223和miR-760水平显著升高,但与HDLC水平或血浆PON-1活性均无相关性。结论高HDLC的CAD患者血浆miR-135a水平明显降低,且与PON-1活性正相关,可能与HDL抗氧化能力有关。Aim To study the correlation between the antioxidantion of plasma high density lipoprotein(HDL)and circulating microRNA(miRNA).Methods Scavenger receptor group B typeⅠdeficient(SR-BⅠ^(-/-))mice as dysfunctional HDL model mice were used to explore the changes of specific miRNA spectrum.From September 2020 to May 2021,30 patients with coronary artery disease(CAD)were enrolled in the Department of Cardiology,Zhongnan Hospital of Wuhan University,non-CAD patients in the same period as control group,both groups with HDL cholesterol(HDLC)higher than 1.04 mmol/L.Results Compared with wild-type mice,the plasma miR-135a in SR-BⅠ^(-/-)mice decreased significantly.Plasma paraoxonase 1(PON-1)activity decreased in CAD patients,PON-1 activity in control group was positively correlated with HDLC level,but not in CAD group.The plasma miR-135a level in CAD patients also decreased,and was positively correlated with PON-1 activity.In CAD group,the level of plasma miR-17 decreased significantly,and the levels of miR-223 and miR-760 increased significantly,but there was no correlation with HDLC level or plasma PON-1 activity.Conclusion The level of plasma miR-135a decreased significantly in CAD patients with high HDLC,and was positively correlated with PON-1 activity,which may be related to the antioxidant function of HDL.

关 键 词：miR-135a 冠状动脉粥样硬化性心脏病 失功能HDL 对氧磷酶1 

分 类 号：R5[医药卫生—内科学]