利用多重PCR和快提DNA结合快速鉴定甜瓜杂种纯度  

Multiplex PCR combines with the rapid DNA extraction to identify the purity of melon hybrids

作　　者：贺玉花[1] 户克云 赵光伟[1] 孔维虎[1] 张健[1] 徐永阳[1] HE Yuhua;HU Keyun;ZHAQ Guangwei;KONG Weihu;ZHANG Jian;XU Yongyang(Zhengzhou Fruit Research Institute of Chinese Academy of Agricultural Sciences,Zhengzhou 450009,Henan,China)

机构地区：[1]中国农业科学院郑州果树研究所,郑州450009

出　　处：《中国瓜菜》2021年第12期23-27,共5页China Cucurbits And Vegetables

基　　金：财政部和农业农村部国家现代农业产业技术体系资助(CARS-25);中国农业科学院科技创新工程(CAAS-ASTIP-2018-ZFRI);河南省科技攻关计划项目(212102110418)。

摘　　要：纯度是决定种子质量的重要指标之一。以众天10号和其父母本为研究材料,将快提DNA法与多重PCR法相结合,成功建立一套三重PCR甜瓜杂种纯度快速鉴定体系。采用碱裂解快速提取DNA的方法,可以在5 min内提取96个DNA;采用三重PCR,能有效克服单一PCR在种子纯度鉴定中速率慢的缺陷。试验中SSR分子鉴定众天10号杂交种纯度为100%,与田间鉴定结果一致。为甜瓜乃至作物杂种种子的纯度鉴定提供了一套精度更高、速度更快、成本更低的技术体系。Purity is one of the most important indicators of seed quality.A hybrid Zhongtian No.10 and their parents were used in this study;and by combining the rapid DNA extraction method with the multiple PCR,a triple PCR purity identification system of hybrid seeds in melon was established.The rapid alkaline lysis method was used to extract the genome DNA,which can extract the genomic DNA of 96 samples in five minutes.The triplex PCR could effectively overcome the shortcomings of the single-PCR with slow speed in seed purity identification.The result of SSR molecular identification was that the purity of the Zhongtian No.10 hybrid was 100%,and it was no significant difference with field identification.This study provides a technical system with the advantages of high-accuracy,rapid and low-cost for the purity identification of hybrid seeds in melon and other crop.

关 键 词：甜瓜 SSR 多重PCR 快提DNA 纯度鉴定 

分 类 号：S652[农业科学—果树学]