Chemical Constituents from Turnip and Their Effects on α-Glucosidase  

作　　者：Swastika Paul Xuemei Zhang Yongping Yang Changan Geng 

机构地区：[1]State Key Laboratory of Phytochemistry and Plant Resources in West China,Yunnan Key Laboratory of Natural Medicinal Chemistry,Kunming Institute of Botany,Chinese Academy of Sciences,Kunming,650201,China [2]Key Laboratory of Biodiversity and Biogeography,Institute of Tibetan Plateau Research at Kunming,Kunming Institute of Botany,Chinese Academy of Sciences,Kunming,650201,China [3]University of Chinese Academy of Sciences,Beijing,100049,China

出　　处：《Phyton-International Journal of Experimental Botany》2020年第1期131-136,共6页国际实验植物学杂志（英文）

基　　金：This work was supported by the Yunnan Science Fund for Excellent Young Scholars,the National Natural Science Foundation of China(81773612);the Youth Innovation Promotion Association,CAS(2013252);the Reserve Talents of Young and Middle-aged Academic and Technical Leaders in Yunnan Province.

摘　　要：Brassica rapa var.rapa(turnip)is an important crop in Qinghai-Tibet Plateau(QTP)with anti-hypoxic effect.Turnip is rich in glucosinolates,isothiocyanates and phenolic compounds with diverse biological activities,involving anti-oxidant,anti-tumor,anti-diabetic,anti-inflammatory,anti-microbial,hypolipidemic,cardioprotective,hepatoprotective,nephroprotective and analgesic properties.In this study,the ethyl acetate(EtOAc)and butanol parts of Brassica rapa were first revealed with inhibitory effects onα-glucosidase,whereas the water part was inactive.Subsequent bioassay-guided isolation on the EtOAc and butanol parts yielded 12 compounds,involving three indole derivatives,indole-3-acetonitrile(1)4-methoxyindole-3-acetonitrile(2)and indole-3-aldehyde(3)two flavonoids,liquiritin(4)and licochalcone A(5)two phenylpropanoids,sinapic acid(6)and caffeic acid(7)two phenylethanol glycosides,2-phenylethylβ-glucopyranoside(8)and salidroside(9)and three other compounds,syringic acid(10)adenosine(11)and(3β,20E)-ergosta-5,20(22)-dien-3-ol(12)Licochalcone A(5)and caffeic acid(7)showedα-glucosidase inhibitory activity with IC 50 values of 62.4±8.0μM and 162.6±3.2μM,comparable to the positive control,acarbose(IC 50=142±0.02μM).Docking study suggested that licochalcone A(5)could well align in the active site ofα-glucosidase(docking score=-52.88)by forming hydrogen bonds(Gln1372,Asp1420,Gln1372,Arg1510),hydrophobic effects(Tyr1251,Tyr1251,Trp1355,Phe1560,Ile1587,Trp1355,Phe1559,Phe1559)andπ-πstacking interaction(Trp1355).This study provides valuable information for turnip as a new resource in searching anti-diabetic candidates.

关 键 词：Brassica rapa TURNIP Α-GLUCOSIDASE licochalcone A docking study 

分 类 号：O62[理学—有机化学]