Production of L-glutamate family amino acids in Corynebacterium glutamicum:Physiological mechanism,genetic modulation,and prospects  

作　　者：Qi Sheng Xiao-Yu Wu Xinyi Xu Xiaoming Tan Zhimin Li Bin Zhang 

机构地区：[1]Jiangxi Engineering Laboratory for the Development and Utilization of Agricultural Microbial Resources,College of Bioscience and Bioengineering,Jiangxi Agricultural University,Nanchang,330045,China [2]State Key Laboratory of Biocatalysis and Enzyme Engineering,School of Life Sciences,Hubei University,Wuhan,430062,China

出　　处：《Synthetic and Systems Biotechnology》2021年第4期302-325,共24页合成和系统生物技术（英文）

基　　金：This work was supported by National Natural Science Foundation of China[No.32000057];Open Funding Project of the State Key Laboratory of Biocatalysis and Enzyme Engineering(SKLBEE2019015);Jiangxi Provincial Natural Science Foundation(No.20202BAB213023);Jiangxi Province Postgraduate Innovation Special Fund Project[No.YC2020-S258].

摘　　要：L-glutamate family amino acids(GFAAs),consisting of L-glutamate,L-arginine,L-citrulline,L-ornithine,L-proline,L-hydroxyproline,γ-aminobutyric acid,and 5-aminolevulinic acid,are widely applied in the food,pharmaceutical,cosmetic,and animal feed industries,accounting for billions of dollars of market activity.These GFAAs have many functions,including being protein constituents,maintaining the urea cycle,and providing precursors for the biosynthesis of pharmaceuticals.Currently,the production of GFAAs mainly depends on microbial fermentation using Corynebacterium glutamicum(including its related subspecies Corynebacterium crenatum),which is substantially engineered through multistep metabolic engineering strategies.This review systematically summarizes recent advances in the metabolic pathways,regulatory mechanisms,and metabolic engineering strategies for GFAA accumulation in C.glutamicum and C.crenatum,which provides insights into the recent progress in L-glutamate-derived chemical production.

关 键 词：L-glutamate family Amino acids C.glutamicum Regulatory mechanism Metabolic engineering FERMENTATION 

分 类 号：Q93[生物学—微生物学]