Deacetoxycephalosporin C synthase(expandase):Research progress and application potential  

作　　者：Xiaofan Niu Jian Zhang Xianli Xue Depei Wang Lin Wang Qiang Gao 

机构地区：[1]Key Laboratory of Industrial Fermentation Microbiology,Ministry of Education,College of Biotechnology,Tianjin University of Science and Technology,Tianjin,300457,China [2]National Demonstration Center for Experimental Bioengineering Education(Tianjin University of Science and Technology),Tianjin,300457,China [3]Tianjin Microbial Metabolism and Fermentation Process Control Technology Engineering Center,Tianjin,300457,China [4]College of Artificial Intelligence,Tianjin University of Science and Technology,Tianjin,300457,China

出　　处：《Synthetic and Systems Biotechnology》2021年第4期396-401,共6页合成和系统生物技术（英文）

基　　金：This work was supported by the Key Project of Natural Science Foundation of Tianjin Municipal Science and Technology Bureau of China(20JCZDJC00140);National Basic Research Program(973 Program)of China(2013CB734004);National Natural Science Foundation of China(31370075,31471725,31902193,61603273);Key Technology R&D Project of Shandong Province of China(Fostering Talent Project)(2016GRC3201).

摘　　要：Cephalosporins play an indispensable role against bacterial infections.Deacetyloxycephalosporin C synthase(DAOCS),also called expandase,is a key enzyme in cephalosporin biosynthesis that epoxides penicillin to form the hexavalent thiazide ring of cephalosporin.DAOCS in fungus Acremonium chrysogenum was identified as a bifunctional enzyme with both ring expansion and hydroxylation,whereas two separate enzymes in bacteria catalyze these two reactions.In this review,we briefly summarize its source and function,improvement of the conversion rate of penicillin to deacetyloxycephalosporin C through enzyme modification,crystallography features,the prediction of the active site,and application perspective.

关 键 词：Deacetyloxycephalosporin C synthase Expandase Streptomyces clavuligerus Acremonium chrysogenum PROGRESS Application 

分 类 号：O64[理学—物理化学]