Analysis of the relationship between bile duct and duodenal microbiota reveals that potential dysbacteriosis is the main cause of primary common bile duct stones  

作　　者：Zhitang Lyu Tingting Yu Lichao Zhang Xiaona Xu Yijun Zhang Jihong Li Zhirong Li Wei Zhang Senlin Hou 

机构地区：[1]School of Life Sciences,Institute of Life Science and Green Development,Hebei University,Baoding,PR China [2]The Second Hospital of Hebei Medical University,Shijiazhuang,PR China [3]Key Laboratory of Microbial Diversity Research and Application of Hebei Province,Baoding,PR China [4]Engineering Laboratory of Microbial Breeding and Preservation of Hebei Province,Baoding,PR China

出　　处：《Synthetic and Systems Biotechnology》2021年第4期414-428,共15页合成和系统生物技术（英文）

摘　　要：Bacteria play an important role in the formation of primary Common Bile Duct(CBD)stones.However,the composition and function of the microbiota of bile duct in patients with primary CBD stones remained to be explored.We utilized the 16S rRNA gene high-throughput sequencing technology to analyze the microbial diversity and community composition of biliary and duodenal microbiota in 15 patients with primary CBD stones and 4 patients without biliary tract diseases.Alpha diversity analysis showed that the microbiota richness was similar in bile and intestinal fluid;Beta diversity analysis showed that there were differences in the composition between biliary microbiota and the duodenal microbiota,but the abundance of the main groups showed similarities.The composition of the biliary microbiota from gallstone patients was more complex,as was the duodenal microbiota.Proteobacteria and Firmicutes were the dominant bacteria at phylum level,accounting for at least 75%of the total reads in each subgroup.Pseudomonas and EscherichiaShigella were the major genus among subgroups,but Escherichia-Shigella had increased abundance in duodenal microbiota with primary choledocholithiasis,which may play an important role in stone formation.It is noteworthy that Clostridiumsensu_stricto,Lachnospiraceae_UCG-008,Butyrivibrio and Roseburia which could produce short chain fatty acids(SCFAs),were significantly decreased in biliary microbiota with primary CBD stones(p<0.05).Our study provided new insights into the compositional of normal biliary microbiota.The micro-ecology of biliary and duodenal in patients with stones is complex and closely related,and there is a potential for dysbacteriosis.The decrease in abundance of certain major acid-producing bacteria affects the health of the biliary tract and thus leads to the formation of stones.

关 键 词：Primary common bile duct stones ERCP DYSBACTERIOSIS Bile duct microbiota Duodenal microbiota 16S rRNA gene high-throughput sequencing Short chain fatty acids 

分 类 号：R657.4[医药卫生—外科学]