基于鲁米诺-白藜芦醇荧光体系检测花生衣中的白藜芦醇和苷  

Detection of Resveratrol and Glycosides in Peanut Coat based on Fluorescence System of Luminol-Resveratrol

作　　者：宋吉英[1] 汪琪琦 王艳丽[1] SONG Ji-ying;WANG Qi-qi;WANG Yan-li(College of Chemistry&Pharmacy,Qingdao Agricultural University,Qingdao 266109,China)

机构地区：[1]青岛农业大学化学与药学院,山东青岛266109

出　　处：《化学试剂》2022年第1期97-101,共5页Chemical Reagents

基　　金：山东省高等学校省级大学生创新创业训练计划项目(S201910435032)。

摘　　要：探讨了鲁米诺-白藜芦醇体系的荧光性质,提取了3种颜色花生衣中的白藜芦醇和苷,并进行测定。实验采用超声-微波协同萃取技术进行提取,正交试验确定最佳提取条件,白藜芦醇和苷的含量用荧光法进行测定。结果表明,花生衣中白藜芦醇和苷提取的最佳试验条件是乙醇作萃取剂、料液比1∶25(g/mL)、微波功率50 W、萃取时间15 min;在pH 9的弱碱性环境下反应20 min,白藜芦醇对鲁米诺的荧光增敏作用最强,在0.5~50μg/mL浓度范围内,白藜芦醇浓度对鲁米诺的荧光增敏作用呈线性关系,由此测定3种颜色(粉、红、黑)的花生衣中白藜芦醇和苷的含量分别为0.261、0.370、0.257 mg/g。该方法可成功用于花生衣中白藜芦醇和苷的分析检测。In this work,the resveratrol and glycosides in three colors peanut coat were extracted and determined.Meanwhile,the fluorescence properties of luminol-resveratrol system were investigated.The ultrasonic-microwave synergistic extraction strategy was successfully used in resveratrol and glycosides extraction from peanut coat.The extraction conditions were optimized by orthogonal tests,the contents of resveratrol and glycosides were detected by fluorescence method.The results indicated that the optimal experimental conditions for extraction of resveratrol and glycosides from peanut coat were obtained when extractant was ethyl alcohol,solid-liquid ratio was 1∶25(g/mL),microwave power was 50 W and extraction time was 15 min.Fluorescence sensitizing effect was enhanced strongest when pH 9 and reaction time was 20 min.The fluorescence sensitizing effect of resveratrol towards luminol exhibited excellent linearly increased trend with the increased concentration of resveratrol ranging from 0.5~50μg/mL.The contents of resveratrol and glycosides in three colors(pink,red and black)peanut coat were 0.261,0.370 and 0.257 mg/g,respectively.The strategy can be successfully employed in the determination of resveratrol and glycosides in peanut coat.

关 键 词：白藜芦醇 鲁米诺 荧光 花生衣 苷 

分 类 号：O657[理学—分析化学]