硃砂烟CYP82E4基因表达模式及烟碱转化率分析  

The CYP82E4 expression pattern and nicotine conversion rate in cherry-red tobacco

作　　者：宋中邦[1] 隋学艺 张谊寒[1] 王亚辉[1] 赵高坤[1] 李勇[1] 王丙武[1] 刘加红 解燕[2] 杨世波 顾华国[1] SONG Zhongbang;SUI Xueyi;ZHANG Yihan;WANG Yahui;ZHAO Gaokun;LI Yong;WANG Bingwu;LIU Jiahong;XIE Yan;YANG Shibo;GU Huaguo(Yunnan Academy of Tobacco Agricultural Sciences,Key Laboratory of Tobacco Biotechnological Breeding and National Tobacco Genetic Engineering Research Center,Kunming 650021,China;Qujing Municipal Company of Yunnan Provincial Tobacco Corporation,Qujing 655000,China;Yunnan Provincial Oriental Tobacco Corporation,Baoshan 678000,China)

机构地区：[1]云南省烟草农业科学研究院/烟草行业烟草生物技术育种重点实验室/国家烟草基因工程研究中心,昆明650021 [2]云南省烟草公司曲靖市公司,曲靖655000 [3]云南香料烟有限责任公司,保山678000

出　　处：《中国烟草学报》2021年第6期75-80,共6页Acta Tabacaria Sinica

基　　金：中国烟草总公司云南省公司科技项目(2020530000241002)。

摘　　要：为解析硃砂烟基本特征特性,以"硃砂2号"为研究材料,调查其田间农艺性状及硃砂烟叶产出规律,通过qPCR分析CYP82E4基因表达模式,并检测硃砂烟叶生物碱含量。结果表明,硃砂2号农艺性状与云烟87无显著差异,烤后硃砂烟叶比率随着叶位增高逐步上升。硃砂2号烟叶中CYP82E4基因高水平表达,导致大量烟碱被转化生成去甲基烟碱,浅红、中等红色、深红三种类型硃砂烟叶的烟碱转化率分别为86.95%、90.86%、93.85%,远高于云烟87的5.12%。硃砂烟子代植株中CYP82E4基因高表达、高烟碱转化率性状能够稳定遗传,烤后烟叶均呈现明显的硃砂烟外观特征。本研究揭示了硃砂烟基本特征特性,为进一步研究硃砂烟的成因及化学特性提供理论支撑。In this study, we investigated the agronomic traits and phenotype rate of the flue-cured leaves of Zhusha2 cherry-red tobacco line. In addition, we also characterized the expression pattern of CYP82E4 gene and nicotine conversion rate(NCR) in Zhusha2. Field trail results showed that there was no significant difference between Zhusha2 and Yunyan87 in agronomic traits. The proportion of the fluecured leaves with featured cherry-red phenotype is gradually increased leaf position from bottom to up. On the other hand, the expression of CYP82E4 gene was dramatically induced in Zhusha2, resulting in enhanced conversion from nicotine to nornicotine, with the NCR being 86.95%, 90.86% and 93.85% in light-red, medium-red, and dark-red cured leaves of Zhusha2 variety, respectively, which were significantly higher than NCR(5.12%) in the Yunyan87 control. Additionally, the high expression of CYP82E4 and high nicotine conversion rate in the flue-cured leaves of the Zhusha2 offspring are beneficial for for stable inheritance from its parental line. Our results provide theoretical support for further study on the genesis and chemical properties of Zhusha tobacco.

关 键 词：樱桃红 硃砂烟 CYP82E4 烟碱转化率 

分 类 号：TS44[农业科学—烟草工业]