小型一体式针尖增强拉曼光谱仪设计与评价  

Design and Evaluation of Integrated Tip-enhanced Raman Spectrometer

作　　者：曾英杰 支瑜亮 范贤光[1,2] 王昕[1,2] ZENG Ying-Jie;ZHI Yu-Liang;FAN Xian-Guang;WANG Xin(Department of Instrumental and Electrical Engineering,Xiamen University,Xiamen 361102,China;Fujian Key Laboratory of Universities and Colleges for Transducer Technology,Xiamen University,Xiamen 361102,China)

机构地区：[1]厦门大学仪器与电气系,厦门361102 [2]厦门大学传感器技术福建省高校重点实验室,厦门361102

出　　处：《分析化学》2022年第1期39-46,共8页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金项目(Nos.21974118,21874113)资助。

摘　　要：针尖增强拉曼光谱技术(TERS)是拉曼光谱与扫描探针显微镜相结合的一种新型分析测量技术。通过增强“热点”处的拉曼信号,可以更深入了解分子组成和结构、反应机理及分子取向。目前,TERS仪多采用分体式设计,结构复杂、体积庞大、价格昂贵,限制了仪器的普及和相关研究的开展。本研究设计并开发了一体式TERS仪,有效地提高了仪器的集成度,降低了光纤的传输损耗,减少了系统的体积及成本。仪器主要由激光激发与拉曼收集模块、光谱仪模块及针尖和激光耦合成像模块构成,仪器的光谱范围为640~770 nm,光学分辨率为0.56 nm,对于Ne-Ar校准光源,观测到的轮廓为高斯线型,四氯化碳的ν(A1)振动峰的轮廓是51%高斯与49%洛伦兹线型的组合。利用此光谱仪成功获取了4′-(pyridine-4-yl)-biphenyl-4-yl)methanethiol(4-PBT)分子的TERS,验证了小型一体式针尖增强拉曼光谱仪的可行性,为针尖拉曼光谱技术发展提供了技术参考。Tip-enhanced Raman spectroscopy(TERS)is a new analytical technology that combines Raman spectroscopy and scanning probe microscopies(SPM).By enhancing the Raman signal at“hot spots”,it is possible to have a deeper understanding of molecular composition and structure,reaction mechanism,and molecular orientation.At present,most of tip-enhanced Raman instruments adopt a split design,which is bulky,expensive and complicated in structure,limiting the popularization of the instrument and the development of related research.In this work,an integrated tip-enhanced Raman spectroscopy instrument was designed and developed,which effectively improved the integration of the instrument,reduced the transmission loss of the optical fiber and reduced the volume and cost of the system.The spectrometer consisted of a laser excitation and Raman collection module,a spectrometer module,and a tip and laser coupled imaging module.The three modules were placed in a closed housing with a volume of 410 mm×305 mm×85 mm.The instrument was optically calibrated using a standard Ne-Ar light source.The resolution of the instrument was 0.56 nm,and the spectral detection range was 640-770 nm.For the Ne-Ar calibration light source,the observed profile was Gaussian lineshape,and the contour of theν(A1)vibration peak of carbon tetrachloride was a combination of 51%Gaussian and 49%Lorentzian lines.Finally,the tip-enhanced Raman spectrum of 4′-(pyridine-4-yl)-biphenyl-4-yl)methanethiol(4-PBT)molecules was successfully obtained using this spectrometer,which verified the feasibility of the integrated spectrometer and provided technical accumulation for the promotion of tip Raman spectroscopy technology.

关 键 词：针尖拉曼增强 仪器设计 光谱标定 拉曼光谱 

分 类 号：TH744.1[机械工程—光学工程]