噻虫嗪胁迫对水稻内生细菌群落结构和多样性的影响  

Effects of thiamethoxam stress on the community composition and diversity of endophytic bacteria in rice

作　　者：黄博闻 崔凯 余向阳 葛静 HUANG Bo-wen;CUI Kai;YU Xiang-yang;GE Jing(School of Environmental and Safety Engineering,Jiangsu University,Zhenjiang 212013,China;Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base,Ministry of Science and Technology,Nanjing 210014,China)

机构地区：[1]江苏大学环境与安全工程学院,江苏镇江212013 [2]省部共建国家重点实验室培育基地-江苏省食品质量安全重点实验室,江苏南京210014

出　　处：《江苏农业学报》2021年第6期1370-1377,共8页Jiangsu Journal of Agricultural Sciences

基　　金：国家自然科学基金项目(31872002、31772197)。

摘　　要：为揭示噻虫嗪对水稻内生细菌群落的影响,采用Illumina HiSeq高通量测序技术对室内盆栽的水稻根、茎、叶内生细菌进行16S rRNA测序。获得用于分析的686889条优质序列和265个操作分类单元(OTU)。通过α多样性分析发现,土壤噻虫嗪处理对根中内生细菌群落多样性影响显著,对茎、叶影响不明显。在属水平上对代表性OTU进行注释,发现水稻各器官中内生细菌组成受噻虫嗪的影响明显,表明噻虫嗪对水稻内生细菌组成结构影响显著。水稻各器官中内生细菌群落组成与噻虫嗪残留浓度有一定相关性,在水稻茎、叶中,相比于低施用量噻虫嗪处理,内生菌群落在高施用量噻虫嗪处理下改变更明显。The effects of thiamethoxam stress on endophytic bacterial communities in roots,stems and leaves of rice were studied by Illumina Hiseq high-throughput sequencing technology with indoor pot culture and soil addition treatment.High quality sequences and operational taxons(OTUs)were 686889 and 265,respectively.The results ofαdiversity index showed that thiamethoxam had a significant effect on the structure and diversity of endophytic bacterial community in rice roots,but had no significant effect on the diversity of endophytic bacterial community in rice stems and leaves.The representative OTUs were annotated at the genus level.It was found that the composition of endophytic bacteria in rice organs was significantly affected by thiamethoxam,and which was in correlation with the residual level of thiamethoxam.The changes of endophytic bacterial community under the treatment with high application rate of thiamethoxam were more obvious than those under the treatment with low application rate of thiamethoxam in the stems and leaves of rice.

关 键 词：新烟碱杀虫剂 噻虫嗪胁迫 水稻内生细菌 高通量测序 

分 类 号：X592[环境科学与工程—环境工程]