徐淮地区7个甘薯茎线虫群体的形态学及分子生物学鉴定  

Morphological and molecular biology identification of seven stem nematode populations of sweet potato in Xuhuai area

作　　者：陈晶伟 马居奎 张成玲[1] 杨冬静[1] 唐伟 谢逸萍[1] 孙厚俊[1] CHEN Jing-wei;MA Ju-kui;ZHANG Cheng-ling;YANG Dong-jing;TANG Wei;XIE Yi-ping;SUN Hou-jun(Xuzhou Institute of Agricultural Sciences in Xuhuai Area of Jiangsu/Key Laboratory of Biology and Genetic Improvement of Sweet Potato of Ministry of Agriculture and Rural Affairs,Xuzhou 221131,China)

机构地区：[1]江苏徐淮地区徐州农业科学研究所/农业农村部甘薯生物学与遗传育种重点实验室,江苏徐州221131

出　　处：《江苏农业学报》2021年第6期1409-1416,共8页Jiangsu Journal of Agricultural Sciences

基　　金：国家现代农业产业技术体系建设专项(CARS-10-B15);徐州市农业科学院基金项目(RC2019002)。

摘　　要：从江苏省徐淮地区7个采集地的甘薯块根中分离到7个甘薯茎线虫群体,通过形态学鉴定,其特征与已报道的腐烂茎线虫(Ditylenchus destructor)基本一致。利用rDNA-内转录间隔区(ITS)通用引物、腐烂茎线虫特异性引物检测,发现群体JSXZ为B(L)型,其余6个分离群体均为A(S)型腐烂茎线虫。结合ITS-5.8S-ITS2区序列分析并构建系统发育树,结果进一步表明,所获序列与腐烂茎线虫相似性最高,且各分离群体与徐淮地区已报道的相同基因型的腐烂茎线虫群体序列相似性均在99%以上。群体JSXZ没有与腐烂茎线虫A型、B型群体聚在一起,而是与C型、D型群体聚为1个分支,且和C型亲缘关系更近,所以群体JSXZ被鉴定为C型腐烂茎线虫;群体JSWJ、JSYJ、JSGS、JSHW、JSLQ和JSNG与A型腐烂茎线虫群体聚为1个分支,均为A型腐烂茎线虫,且与其他基因型区分明显。危害徐淮地区甘薯的腐烂茎线虫亲缘关系近,基因型种类多,存在A型、B型和C型,其中A型腐烂茎线虫为优势群体。Seven populations of sweet potato stem nematodes were isolated from storage roots of sweet potatoes from seven regions in Xuhuai area of Jiangsu province.Characteristics of the stem nematodes were basically identical to Ditylenchus destructor reported previously through morphological identification.By detections using universal primers of rDNA-internal transcribed spacer(ITS)region and specific primers of D.destructor,it was found that population JSXZ belonged to B(L)type of D.destructor,while the other six isolated populations all belonged to A(S)type of D.destructor.The results further suggested that,the sequences of the stem nematodes shared the highest similarity with D.destructor though sequences analysis of ITS-5.8S-ITS2 region and phylogenetic tree construction,and sequence similarity of the separated populations compared with the D.destructor populations carrying the same genotype reported in Xuhuai area were all above 99%.Results of phylogenetic tree analysis showed that population JSXZ did not cluster into one branch with type A and type B populations of D.destructor,but clustered into one branch with type C and type D populations of D.destructor,and had closer affinity with type C.Therefore,JSXZ population from the sweet potato was confirmed as type C of D.destructor.Populations of JSWJ,JSYJ,JSGS,JSHW,JSLQ and JSNG were clustered into one branch with population of type A of D.destructor,which were all confirmed as type A of D.destructor and were clearly distinguished from other genotypes.It was indicated that populations of D.destructor which harmed sweet potatoes in Xuhuai area were closely related and had various genotypes,such as type A,type B and type C,among which type A was the dominant population.

关 键 词：甘薯 腐烂茎线虫 鉴定 系统发育树 

分 类 号：S432.45[农业科学—植物病理学]