养殖水无机氮盐测定方法比较分析研究  

Comparative studies of different detection methods of nitrogen in aquaculture water

作　　者：马晓娜[1,2,3] 李碧莹 吴乐乐[1] 李贤 李军 MA Xiao-na;LI Bi-ying;WU Le-le;LI Xian;LI Jun(CAS Key Laboratory of Experimental Marine Biology,Institute of Oceanology,Chinese Academy of Sciences,Qingdao 266071,China;Marine Biology and Biotechnology Laboratory,National Laboratory for Marine Science and Technology Pilot(Qingdao),Qingdao 266071,China;Center for Ocean Mega-Science,Chinese Academy of Sciences,Qingdao 266071,China;College of Environmental Science and Engineering,Qingdao University,Qingdao 266071,China)

机构地区：[1]中国科学院海洋研究所中国科学院实验海洋生物学重点实验室,山东青岛266071 [2]青岛海洋科学与技术国家实验室海洋生物学与生物技术功能实验室,山东青岛266071 [3]中国科学院海洋大科学研究中心,青岛市266071 [4]青岛大学环境科学与工程学院,山东青岛266071

出　　处：《海洋湖沼通报》2021年第6期117-123,共7页Transactions of Oceanology and Limnology

基　　金：山东省重大科技创新工程专项(2018SDKJ0502-2);国家海水鱼产业技术体系(CARS-47);广东省重点研发计划(2019B020215001);山东省农业重大应用技术创新项目(SD2019YY011);山东省2018年度农业重大应用技术创新项目(2019JZZY020710)。

摘　　要：养殖水体中的无机氮营养盐与养殖生物的健康生长息息相关,目前存在多种无机氮盐检测方法,准确定量不同盐度养殖水水质状况的方法缺失。本实验采用不同氨氮(TAN)检测方法(纳氏试剂分光光度法、改良纳氏试剂分光光度法、靛酚蓝分光光度法)、亚硝酸盐氮(NO_(2)^(-)-N)检测方法(盐酸萘乙二胺分光光度法、分子吸收分光光度法)、硝酸盐氮(NO_(3)^(-)-N)检测方法(紫外分光光度法、锌-镉还原法)分别对不同盐度水体中TAN、NO_(2)^(-)-N、NO_(3)^(-)-N含量进行检测,从而筛选出适合不同盐度养殖水体的检测方法。实验结果表明:盐度显著影响TAN和NO_(3)^(-)-N浓度测量,对NO_(2)^(-)-N的测量无显著影响;海水养殖水中TAN的测定推荐稀释到盐度4,使用改良纳氏试剂比色法进行测量,回收率在94.5%~113.5%;NO_(2)^(-)-N的测量推荐使用萘乙二胺分光光度法,回收率在86.5%~97.34%;盐度大于18的水中NO_(3)^(-)-N的测定推荐直接使用锌镉还原法进行测量,回收率在102.63%~120.23%,其他盐度养殖水可稀释至4使用紫外分光光度法测量,回收率在107.56%~126.75%。本研究结果可为养殖水环境监测和定向调控提供参考依据。The concentration of nitrogen in aquaculture water is closely related to the health of the cultured species.There are various nitrogen detection methods,however,studies on the standard detection methods in aquaculture water of different salinities are limited and inconclusive.In the study,three ammonium detection methods(Nessler′s reagents spectrophotometric method,improved Nessler′s reagents spectrophotometric method,indophenol blue spectrophotometric method),two nitrite detection methods(ethylenediamine dihydrochloride spectrophotometric method,molecular absorption spectrometry),two nitrate detection methods(ultraviolet spectrophotometric method,zinc cadmium reduction method)were applied to choose the appropriate detection method.Results showed that salinity significantly affected the detection of ammonium and nitrate.Based on the results,improved Nessler′s reagents spectrophotometric method was recommended to detect ammonium in the aquaculture water when diluted to salinity 4 with the recovery rate ranged from 94.5%to 113.5%.Ethylenediamine dihydrochloride spectrophotometric method was recommended to detect nitrite in the aquaculture water with the recovery rate varied between 86.5%and 97.34%.Zinc cadmium reduction method was recommended to detect nitrate in the aquaculture water if salinity≥18 with the recovery rate ranged from 102.63%to 120.23%,and otherwise,ultraviolet spectrophotometric method was recommended to detect nitrate in the aquaculture water which diluted to salinity 4.This study provided a reference to the monitoring and regulation of aquaculture water.

关 键 词：水产养殖 盐度 氨氮 亚硝酸盐氮 硝酸盐氮 

分 类 号：S912[农业科学—水产科学]