Autographa Californica Multiple Nucleopolyhedrovirus orf13 Is Required for Efficient Nuclear Egress of Nucleocapsids  

作　　者：Xingang Chen Xiaoqin Yang Chengfeng Lei Fujun Qin Xiulian Sun Jia Hu 

机构地区：[1]Wuhan Institute of Virology,Center for Biosafety MegaScience,Chinese Academy of Sciences,Wuhan 430071,China [2]University of Chinese Academy of Sciences,Beijing 100049,China

出　　处：《Virologica Sinica》2021年第5期968-980,共13页中国病毒学（英文版）

基　　金：supported by the National Key Research and Development Program of China(2017YFD0201206);the WIV “One-Three-Five”strategic program(Y602111SA1 to XS)。

摘　　要：Autographa californica multiple nucleopolyhedrovirus(Ac MNPV)orf13(ac13)is a conserved gene in all sequenced alphabaculoviruses.However,its function in the viral life cycle remains unknown.In this study,we found that ac13 was a late gene and that the encoded protein,bearing a putative nuclear localization signal motif,colocalized with the nuclear lamina.Deletion of ac13 did not affect viral genome replication,nucleocapsid assembly or occlusion body(OB)formation,but reduced virion budding from infected cells by approximately 400-fold compared with the wild-type virus.Deletion of ac13 substantially impaired the egress of nucleocapsids from the nucleus to the cytoplasm,while the OB morphogenesis was unaffected.Taken together,our results indicated that ac13 was required for efficient nuclear egress of nucleocapsids during virion budding,but was dispensable for OB formation.

关 键 词：Autographa californica multiple nucleopolyhedrovirus(AcMNPV) orf13 Nucleocapsid egress OB morphogenesis 

分 类 号：Q939.4[生物学—微生物学]