Re-isolation of Wuxiang Virus from Wild Sandflies Collected from Yangquan County, China  

作　　者：Qinyan Wang Shihong Fu Jingxia Cheng Xiuyan Xu Jing Wang Bin Wu Xiaodong Tian Yan Li Ying He Fan Li Kai Nie Songtao Xu Bin Wang Huanyu Wang Xiaoqing Lu Guodong Liang 

机构地区：[1]Qingdao University,Qingdao 266071,China [2]State Key Laboratory of Infectious Disease Prevention and Control,National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China [3]Shanxi Province Center for Disease Control and Prevention,Taiyuan 030001,China [4]Yangquan Center for Disease Control and Prevention,Yangquan 045000,China

出　　处：《Virologica Sinica》2021年第5期1177-1186,共10页中国病毒学（英文版）

基　　金：supported by the Ministry of Science and Technology of the People’s Republic of China(2018ZX10711001,2018ZX10734-404-003);National Key Research and Development Program(No.2018YFA0900800);National Science and Technology Major Project(2018ZX10102001);National Natural Science Foundation of China(31900156);Key Research and Development(R&D)Projects of Shanxi Province,China(201803D31205);the United States National Institutes of Health U01 AI151810;Development Grant of the State Key Laboratory of Infectious Disease Prevention and Control(2014SKLID103,2015SKLID505)。

摘　　要：We previously isolated a new species of the genus Phlebovirus from wild sandflies collected from Wuxiang County in central China,which named the Wuxiang virus(WUXV).In this study,we re-isolated the WUXV from wild sandflies collected from two villages in Yangquan County,China in 2019.Four virus isolates that caused cytopathic effects in BHK-21 cells were successfully isolated from sandfly specimens collected from chicken pens and sheep pens.Phylogenetic analyses of the L,M and S gene segments of the viruses revealed that the four virus strains represented the previously isolated WUXV.The minimum infection rate(MIR)of the virus isolated from the sheep pen was 3.21,and the MIR of the virus isolated from the chicken pen was 3.45.The positive rates of Wuxiang virus neutralizing antibodies in serum samples of local healthy people and domestic chickens were 8.7%(4/46)and 100%(4/4),respectively,suggesting that Wuxiang virus can infect human and animal.In view of the fact that Wuxiang virus is infectious to humans and animals and has a relatively wide geographical distribution in China,it is of great public health significance to strengthen the investigation and study on the infection status of Wuxiang virus in humans and animals.

关 键 词：SANDFLY PHLEBOVIRUS Wuxiang virus(WUXV) 

分 类 号：R373.3[医药卫生—病原生物学]