The Functional Characterization of Bat and Human P[3] Rotavirus VP8*s  

作　　者：Dandi Li Mengxuan Wang Tongyao Mao Mingwen Wang Qing Zhang Hong Wang Lili Pang Xiaoman Sun Zhaojun Duan 

机构地区：[1]National Health Commission Key Laboratory for Medical Virology and Viral Diseases,Beijing 102206,China [2]National Institute for Viral Disease Control and Prevention,China CDC,Beijing 102206,China

出　　处：《Virologica Sinica》2021年第5期1187-1196,共10页中国病毒学（英文版）

基　　金：the participation of the ProteinGlycan Interaction Resource of the CFG(supporting grant R24 GM098791);the National Center for Functional Glycomics(NCFG)at Beth Israel Deaconess Medical Center,Harvard Medical School(supporting grant P41 GM103694);supported by grants from the National Natural Science Foundation of China(NSFC)(No.21934005);National Key Research and Development Program of China(2018YFC1200602)。

摘　　要：P[3]rotavirus(RV)has been identified in many species,including human,simian,dog,and bat.Several glycans,including sialic acid,histo-blood group antigens(HBGAs)are reported as RV attachment factors.The glycan binding specificity of different P[3]RV VP8*s were investigated in this study.Human HCR3 A and dog P[3]RV VP8*s recognized glycans with terminal sialic acid and hemagglutinated the red blood cells,while bat P[3]VP8*showed neither binding to glycans nor hemagglutination.However,the bat P[3]VP8*mutant of C189 Y obtained the ability to hemagglutinate the red blood cells,while human P[3]HCR3 A/M2-102 mutants of Y189 C lost the ability.Sequence alignment and structural analysis indicated that residue 189 played an important role in the ligand recognition and may contribute to the cross-species transmission.Structural superimposition exhibited that bat P[3]VP8*model was quite different from the simian P[3]Rhesus rotavirus(RRV)P[3]VP8*,indicating that bat P[3]RV was relatively distinct and partially contributed to the no binding to tested glycans.These results promote our understanding of P[3]VP8*/glycans interactions and the potential transmission of bat/human P[3]RVs,offering more insight into the RV infection and prevalence.

关 键 词：Bat rotavirus VP8* Glycan binding specificity HEMAGGLUTINATION Sialic acid 

分 类 号：R373[医药卫生—病原生物学]