Development of RNA Polymerase Ⅲ-Driven Reverse Genetics System for the Rescue of a Plant Rhabdovirus  

作　　者：Xiaoyan Zhang Kai Sun Yan Liang Chenglu Zhao Zhenghe Li 

机构地区：[1]State Key Laboratory of Rice Biology,Institute of Biotechnology,Zhejiang University,Hangzhou 310058,China [2]Zhejiang Provincial Key Laboratory of Biometrology and Inspection&Quarantine,College of Life Sciences,China Jiliang University,Hangzhou 310018,China [3]Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insect Pests,Zhejiang University,Hangzhou 310058,China [4]Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province,Zhejiang University,Hangzhou 310058,China

出　　处：《Virologica Sinica》2021年第5期1252-1255,共4页中国病毒学（英文版）

基　　金：supported by National Natural Science Foundation of China(Grant No.31671996);Natural Science Foundation of Zhejiang Province,China(Grant No.LZ20C140004);National Key R&D Program of China(No.2019YFD1001800)。

摘　　要：Dear Editor,De novo generation of negative-stranded RNA viruses(NSVs)requires efficient transcription of integral viral RNAs with precise termini from cloned plasmids.Studies during the past 25 years with animal NSVs have established the bacteriophage T7 RNA polymerase(Pol)-and endogenous Pol I-based transcription systems as the most efficient platforms for recovery of recombinant NSVs(Bridgen 2012).Unfortunately.

关 键 词：ENDOGENOUS RESCUE PRECISE 

分 类 号：S432.41[农业科学—植物病理学]