流式细胞分析的规范化和标准化  

Standardization and normalization of flow cytometry

作　　者：屈晨雪[1] 王建中[1] Qu Chenxue;Wang Jianzhong(Department of Clinical Laboratory,Peking University First Hospital,Beijing 100034,China)

机构地区：[1]北京大学第一医院检验科,北京100034

出　　处：《中华检验医学杂志》2021年第12期1105-1108,共4页Chinese Journal of Laboratory Medicine

摘　　要：流式细胞分析的临床应用日益广泛。由于流式细胞分析强大的用户自定义功能、不同原理的仪器配置差异,加之缺乏标准品等诸多因素,导致结果可比性有待提高。因此,流式细胞分析面临规范化和标准化这一巨大挑战。规范操作过程包括标本染色和离心的规范化、合理选择抗体和组合、标准化仪器检测以及数据分析等将极大促进流式细胞分析的规范化和标准化工作进展。Flow cytometry has been widely used in clinical practice.Due to the powerful user-defined function of flow cytometry,different instrument settings among manufacturers and lack of standard materials,the comparability of results needs to be improved and flow cytometry is facing the great challenge of normalization and standardization.In this paper,the recent progress of normalization and standardization about flow cytometry was discussed form the aspects of standardizing operation protocol,including specimen and centrifugation,standardizingantibody selection and panel combination and ensuring instrument and data analysis consistency.

关 键 词：流式细胞分析 标准化 规范化 

分 类 号：R446.11[医药卫生—诊断学]