一株拮抗性多粘类芽孢杆菌的鉴定及其对小麦赤霉病的田间防效  

Identification of Paenibacillus polymyxa as antagonist to Fusarium head blight of wheat and its field control efficacy

作　　者：邓云 DENG Yun(Nanping Institute of Agricultural Sciences,Nanping,Fujian 354200,China)

机构地区：[1]福建省南平市农业科学研究所,福建南平354200

出　　处：《福建农林大学学报（自然科学版）》2022年第1期21-26,共6页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：国家重点研发计划项目(2017YFD0101000).

摘　　要：采用平板对峙法从长期开展小麦赤霉病抗性鉴定的鉴定圃土壤中分离筛选出一株具有广谱抗真菌活性的微生物菌株DY04.结合菌落形态观察和16S rDNA基因序列分析,将其鉴定为多粘类芽孢杆菌.通过灌根、穗部喷洒原菌液和穗部喷洒发酵液等3种施药方法研究多粘类芽孢杆菌菌株DY04对小麦赤霉病的防效和小麦产量的变化.结果表明:将该菌株灌根处理可有效防治小麦赤霉病,防治率达58.43%;同时,小麦籽粒千粒重比对照增加8.83%,理论产量增加45.21%.A microbial strain DY04 with broad-spectrum antifungal activity was isolated and screened using plate confrontation method from nursery soil where long-term study on the resistance of Fusarium head blight(FHB)of wheat was carried out.The strain was identified as Paenibacillus polymyxa by colony morphology analysis and 16S rDNA gene sequencing.Three field application methods,namely root irrigation,spraying with viable bacteria on panicle and spraying with fermented liquid on panicle,were used to assess the control effect of DY04 on FHB and wheat yield.The results showed that root irrigation can effectively control FHB,resulting in a maximum control rate at 58.43%;thousand-grain weight of wheat was 8.83%higher in root irrigation group than that of the control,leading to 45.21%increase in the theoretical yield.

关 键 词：多粘类芽孢杆菌DY04 小麦赤霉病 生物防治 

分 类 号：S482[农业科学—农药学] S481.9[农业科学—植物保护]