龙眼DlTRF2-like的克隆及表达模式分析  

Cloning and Expression Pattern Analysis of DlTRF2-like in Longan

作　　者：李浩然 张树伟[2] 王金英 黄小雄 彭宏祥[2] 徐炯志 潘介春 朱建华[2] 谭春露 李平 丁峰[1,3] LI Haoran;ZHANG Shuwei;WANG Jinying;HUANG Xiaoxiong;PENG Hongxiang;XU Jiongzhi;PAN Jiechun;ZHU Jianhua;TAN Chunlu;LI Ping;DING Feng(Guangxi Academy of Agricultural Sciences/Guangxi Crop Genetic Improvement and Biotechnology Laboratory,Nanning,Guangxi 530007,China;Institute of Horticulture,Guangxi Academy of Agricultural Sciences,Nanning,Guangxi 530007,China;College of Agriculture,Guangxi University/State Key Laborartory for Coservation and Utilizaztion of Subtropical Agro-bioresources,Nanning,Guangxi 530004,China;Agricultural Technology Extension Station of Madong,Guiping,Guangxi 537200,China)

机构地区：[1]广西壮族自治区农业科学院/广西作物遗传改良生物技术重点开放实验室,广西南宁530007 [2]广西壮族自治区农业科学院园艺研究所,广西南宁530007 [3]广西大学农学院/亚热带农业生物资源保护与利用国家重点实验室,广西南宁530004 [4]桂平麻垌镇农业技术推广站,广西桂平537200

出　　处：《热带作物学报》2021年第12期3471-3476,共6页Chinese Journal of Tropical Crops

基　　金：“广西八桂青年学者”专项经费(No.2019AQ01);广西农业科学院基本科研业务专项(桂农科2021YT124);广西作物遗传改良重点实验室开放课题(No.20-065-39-01-05)。

摘　　要：以龙眼成熟叶片为材料,克隆龙眼MYB-related基因家族TRF2-like基因,命名为DlTRF2-like,并对其进行生物信息学和表达模式分析。DlTRF2-like基因属于MYB-related家族的TRF-like亚家族,ORF长度是909 bp,编码含有302个氨基酸残基的蛋白,预测该基因定位于细胞核,同源基因进化分析表明龙眼DlTRF2-like与阿月浑子PvTRF2-like亲缘关系最近。利用实时荧光定量PCR检测DlTRF2-like基因在‘四季蜜’龙眼不同组织及乙烯利和多效唑处理后不同时间的相对表达量。结果表明:DlTRF2-like在龙眼不同组织中均有表达,在花芽中表达量最高;乙烯利和多效唑处理后,DlTRF2-like基因的表达量明显高于对照,推测DlTRF2-like响应乙烯利和多效唑信号促进龙眼成花。The TRF2-like gene of MYB-related family was cloned from the mature leaves of longan and named DLTRF2-like.Bioinformatics and the expression pattern of DLTRF2-like were analyzed.DLTRF2-like gene belongs to the TRF-like subfamily of MYB-related family,with an ORF length of 909 bp,encoding a protein of 302 amino acids which was predicted to be located in the nucleus.Evolution analysis of homologous genes indicated that DLTRF2-like was most closely related to PvTRF2-like in Pistacia vera L.The expression pattern of DLTRF2-like in different tissues and leaves treated by ethephon and paclobutrazol was analyzed by real-time quantitative PCR.The results showed that DLTRF2-like was expressed in all the samples,and the highest expression level was found in flower buds.The expression level of DLTRF2-like gene in the leaves treated by ethephon and paclobutrazol was significantly higher than that of the control.The results indicated that DLTRF2-like might response to ethephon and paclobutrazol signal to promote the flowering of longan.

关 键 词：龙眼 MYB-related 龙眼成花 TRF-like亚家族 表达模式分析 

分 类 号：S667.2[农业科学—果树学]