EBNA1对EBV相关胃癌细胞中E2F1表达的影响  

EFFECT OF EBNA1 ON E2F1 EXPRESSION IN EBV-ASSOCIATED GASTRIC CARCINOMA CELLS

作　　者：宋慧[1] 秦倪 于彩霞 师朵 刘雯[1] SONG Hui;QIN Ni;YU Caixia;SHI Duo;LIU Wen(Department of Pathogenic Biology, School of Basic Medicine, Qingdao University, Qingdao 266071, China)

机构地区：[1]青岛大学基础医学院病原生物学系,山东青岛266071

出　　处：《青岛大学学报（医学版）》2021年第6期860-864,共5页Journal of Qingdao University(Medical Sciences)

基　　金：山东省自然科学基金资助项目(ZR2017BH106)。

摘　　要：目的探讨EB病毒(EBV)相关胃癌(EBVaGC)细胞中EBV编码的核抗原1(EBNA1)对E2F转录因子1(E2F1)表达的调控作用,并分析EBNA1和E2F1在胃癌细胞的表达情况。方法应用实时荧光定量PCR(qRT-PCR)以及Western blotting方法,检测EBVaGC和EBV阴性胃癌(EBVnGC)细胞中EBNA1和E2F1的mRNA和蛋白表达水平;选取两种EBVnGC细胞BGC823和SGC7901,建立EBNA1稳定表达的细胞模型,命名为BGC823-EBNA1和SGC7901-EBNA1,采用qRT-PCR方法和Western blotting方法检测两种细胞中E2F1 mRNA和蛋白表达水平。结果qRT-PCR和Western blotting结果显示,EBNA1只在EBVaGC细胞中表达。EBVaGC细胞的E2F1 mRNA表达水平明显高于EBVnGC细胞,差异有统计学意义(t=13.60,P<0.01);而两种细胞的E2F1蛋白表达水平差异无显著性(P>0.05)。在EBVnGC细胞中稳定过表达EBNA1后E2F1的mRNA和蛋白表达明显上调。结论EBV编码EBNA1诱导E2F1表达上调,提示E2F1可能在EBVaGC的发生发展中起着重要作用。靶向EBNA1/E2F1途径可能为EBVaGC病人的治疗提供新的治疗靶点。Objective To explore the regulatory effect of Epstein-Barr nuclear antigen 1(EBNA1)encoded by Epstein-Barr virus(EBV)on the expression of E2F transcription factor 1(E2F1)in EBV-associated gastric carcinoma(EBVaGC)cells,and to analyze the expression of EBNA1 and E2F1 in gastric carcinoma cells.Methods Quantitative real-time PCR(qRT-PCR)and Western blotting were used to determine the mRNA and protein expression levels of EBNA1 and E2F1 in EBVaGC and EBV-negative gastric carcinoma(EBVnGC)cells.Two types of EBVnGC cells,BGC823 and SGC7901,were selected to establish cell models of stable EBNA1 expression,which were named BGC823-EBNA1 and SGC7901-EBNA1,respectively;qRT-PCR and Western blotting were used to determine the mRNA and protein expression levels of E2F1 in the cells.Results The qRT-PCR and Western blotting results showed that EBNA1 was expressed only in EBVaGC cells.The mRNA expression level of E2F1 in EBVaGC cells was significantly higher than that in EBVnGC cells(t=13.60,P<0.01),while there was no significant difference in E2F1 protein expression level between the two types of cells(P>0.05).After stable EBNA1 overexpression in EBVnGC cells,the mRNA and protein expression levels of E2F1 were significantly upregulated.Conclusion EBV-encoded EBNA1 induces upregulation of E2F1 expression,which suggests that E2F1 may play an important role in the development and progression of EBVaGC.Targeting the EBNA1/E2F1 pathway may provide a new therapeutic target for EBVaGC patients.

关 键 词：疱疹病毒4型 人 胃肿瘤 EBV编码的核抗原1 E2F转录因子1 

分 类 号：R373.9[医药卫生—病原生物学]