不同加工干燥方法对白芍药材中化学成分的影响研究  

Effects and evaluation of different processing and drying methods on components in Paeoniae Radix Alba

作　　者：赵秋龙 杨沛文 钱大玮[1] 卞晓坤 王艺霏 朱振华 郭盛 王团结[3] 严辉[1] 段金廒 ZHAO Qiu-long;YANG Pei-wen;QIAN Da-wei;BIAN Xiao-kun;WANG Yi-fei;ZHU Zhen-hua;GUO Sheng;WANG Tuan-jie;YAN Hui;DUAN Jin-ao(Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization/Jiangsu Provincial Key Laboratory for High Technology Research of Traditional Chinese Medicine Formulae,Nanjing University of Chinese Medicine,Nanjing 210023,China;National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine,Nanjing University of Chinese Medicine,Nanjing 210023,China;Kanion Pharmaceutical Co.,Ltd.,Lianyungang 222001,China)

机构地区：[1]南京中医药大学江苏省中药资源产业化过程协同创新中心/江苏省方剂高技术研究重点实验室,江苏南京210023 [2]中药资源产业化与方剂创新药物国家地方联合工程研究中心,江苏南京210023 [3]江苏康缘药业股份有限公司,江苏连云港222001

出　　处：《中国中药杂志》2021年第22期5839-5847,共9页China Journal of Chinese Materia Medica

基　　金：现代农业技术体系建设专项(CARS-21);国家中药标准化项目(ZYBZH-C-JS-28)。

摘　　要：通过化学成分含量测定,比较不同加工干燥方法对白芍药材中化学成分的影响。收集安徽亳州的新鲜白芍药材,经不同加工方式(煮制、去皮)、不同干燥方式(热风、红外、微波干燥)及不同干燥温度(40、50、60、70℃)处理后,采用高效液相色谱-三重四极杆串联质谱法(ultraperformance liquid chromatography coupled to triple quadrupole with electrospray tandem mass spectrometry,UPLC-TQ-MS)技术,对白芍中单萜苷类、多酚类、鞣质及苯甲酸等11个成分进行含量测定。利用偏最小二乘判别分析(partial least squares discriminant analysis,PLS-DA)及正交偏最小二乘判别分析(orthogonal partial least squares discriminant analysis,OPLS-DA)模型对不同加工干燥后样品化学成分含量进行分析,并通过阈值(variable important in projection,VIP)图评价差异成分贡献率。结果显示,煮制前后、去皮前后及不同干燥方式后的样品均能区分开,芍药内酯苷、没食子酸、五没食子酰葡萄糖及苯甲酸为煮制后的共有差异成分;苯甲酸为去皮后的共有差异成分;没食子酸为不同干燥方式下的共有差异成分;不同温度干燥后,样品不能区分开,没有共有差异成分。该研究可为白芍药材产地干燥加工方法的选择及工艺参数的优化提供参考。The present study evaluates different processing and drying methods and investigates their effects on the chemical components in Paeoniae Radix Alba via content determination.The fresh medicinal materials of Paeoniae Radix Alba collected from Bozhou of Anhui province were processed(boiled and peeled)and dried(hot air-dried,infrared-dried,and microwave-dried)at different temperatures(40,50,60 and 70℃),and the 11 components(monoterpene glycosides,polyphenols,tannin,and benzoic acid)in Paeoniae Radix Alba were determined by ultra-performance liquid chromatography coupled to triple quadrupole with electrospray tandem mass spectrometry(UPLC-TQ-MS).Then the compounds in processed and dried samples were analyzed by partial least squares discriminant analysis(PLS-DA)and orthogonal partial least squares discriminant analysis(OPLS-DA),and the contribution rates of differential components were evaluated by variable important in projection(VIP).The results indicated that the samples obtained by different processing and drying methods could be distinguished.Albiflorin,gallic acid,1,2,3,4,6-pentakis-O-galloyl-β-D-glucose,and benzoic acid were the common differential components in boiled Paeoniae Radix Alba.Benzoic acid was the common differential component in peeled Paeoniae Radix Alba.Gallic acid was the common differential component in Paeoniae Radix Alba dried by different methods.The samples could not be distinguished after drying at different temperatures due to the lack of common differential components.This study is expected to provide a reference for the selection of processing and drying methods and the optimization of processing parameters.

关 键 词：白芍 加工干燥方法 化学成分 偏最小二乘判别分析 正交偏最小二乘判别分析 

分 类 号：R284.1[医药卫生—中药学]