Efficacy of Renshen(Radix Ginseng) plus Fuzi(Radix Aconiti Lateralis Preparata) on myocardial infarction by enhancing autophagy in rats  

作　　者：YANG Liang HUANG Guangyao WANG Yuguang HAN Baoqi ZHENG Bin ZHU Jinmiao GAO Shan GAO Yue 

机构地区：[1]Pharmacology and Toxicology Laboratory,Beijing Institution of Radiation Medicine,Beijing 100850,China [2]Hefei Normal University,School of Chemistry and Chemical Engineering,Hefei 230601,China [3]Department of Pharmacy,Cancer Hospital,Chinese Academy of Sciences,Hefei 230031,China [4]School of Basic Medicine,Anhui Medicial University,Hefei 230032,China

出　　处：《Journal of Traditional Chinese Medicine》2021年第6期909-918,共10页中医杂志（英文版）

基　　金：Supported by National Natural Science Foundation of China(No.81630131,81673633);High-level Talents Research Project of Hefei Normal University(No.2020rcjj30);Key Project of Provincial Scientific Research Platform of Hefei Normal University in 2020(No.2020PTZD14);School Level Quality Engineering Project Of Hefei Normal University(2020szjy10);Undergraduate Innovation and Entrepreneurship Training Program of Hefei Normal University(S202014098050);Key Research and Development Projects of Anhui Province(202004A07020020)。

摘　　要：OBJECTIVE:To elucidate the protective effects of Renshen(Radix Ginseng)and Fuzi(Radix Aconiti Lateralis Preparata)on myocardial infarction(MI)through regulating myocardial autophagy.METHODS:Thirty-one male Sprague-Dawley rats were randomized into five groups(n=6 or 7 for each).After treatment for 3 weeks,electrocardiogram(ECG)and cardiac function were recorded.Hematoxylin and eosin(HE)staining was used to detect pathological changes in the heart.Enzyme-linked immunosorbent assay(ELISA)was used to detect serum B-type brain natriuretic peptide(BNP),cardiac troponin T(c Tn T),tumor necrosis factor-α(TNF-α),and serum inflammatory cytokines.Metabolomic analysis was used to identify differential biomarkers of MI in rats.Immunohistochemistry and western blotting were used to detect BNP,cTnT,TNF-α,LC3B,Beclin-1,p62,and adenosine monophosphate activated protein kinase(AMPK)expression in cardiac tissue.RESULTS:Fuzi(Radix Aconiti Lateralis Preparata)alone or Renshen(Radix Ginseng)plus Fuzi(Radix Aconiti Lateralis Preparata)markedly ameliorated cardiac dysfunction and abnormal ECGs,demonstrated by decreases in the heart weight/body weight ratio,BNP,and c Tn T.Pro-inflammation cytokine interleukin(IL)-1αsignificantly decreased and anti-inflammatory cytokine IL-10 significantly increased in Renshen(Radix Ginseng)single or Renshen(Radix Ginseng)plus Fuzi(Radix Aconiti Lateralis Preparata)groups compared with the control group.HE results suggested that co-treatment produced a greater reduction in cardiomyocyte cross-sectional area than Renshen(Radix Ginseng)or Fuzi(Radix Aconiti Lateralis Preparata)alone.Renshen(Radix Ginseng)plus Fuzi(Radix Aconiti Lateralis Preparata)reversed these changes to different degrees in MI rats.Furthermore,Renshen(Radix Ginseng)plus Fuzi(Radix Aconiti Lateralis Preparata)down-regulated LC3 B,Beclin-1,and AMPK expression in cardiac tissue and upregulated p62 expression.CONCLUSIONS:Renshen(Radix Ginseng)plus Fuzi(Radix Aconiti Lateralis Preparata)may have a greater effect on heart injury induced by

关 键 词：myocardial infarction AUTOPHAGY inflammatory CYTOKINES Renshen(Radix Ginseng) Fuzi(Radix Aconiti Lateralis Preparata) 

分 类 号：R285.5[医药卫生—中药学]