Anti-cataract effects of Dajizhi(Euphorbium) eye drops on selenite-induced cataracts in rats  

作　　者：TANG Chengfang GAO Yang Gulibairemu Yusuyin MAO Yan LI Yujun WANG Yandong GU Zhengyi 

机构地区：[1]Key Laboratory of Xinjiang Phytomedicine Resource and Utilization for Ministry of Education,School of Pharmacy,Shihezi University,Shihezi 832000,China [2]Xinjiang Institute of Materia Medica,Urumqi,830002,China [3]Department of Pharmacy&State Key Laboratory of Ophthalmology,Zhongshan Ophthalmological Center,Sun Yat-sen University,Guangzhou 510060,China [4]College of Pharmacy,Xinjiang Medical University,Urumqi 830011,China [5]Pharmaceutical analysis laboratory,Xinjiang Institute of Materia Medica,Urumqi 830002,China

出　　处：《Journal of Traditional Chinese Medicine》2021年第5期747-752,共6页中医杂志（英文版）

基　　金：Supported by the Major New Drug Development Project of the 13th Five-Year Plan(Study on the Key Technologies for Efficient Extraction of Active Tetracyclic Triterpenes,2017ZX0901003-001-004)。

摘　　要：OBJECTIVE:To evaluate the effects of Dajizhi(Euphorbium)on selenite-induced cataracts.METHODS:Wistar rat pups were divided into 9 groups.Rats in group 1 were subcutaneously injected with saline,and rats in the other groups were injected with sodium-selenite.Every right eye was treated with 5μL eye drops 3 times per day,and the left eye received no treatment.The eyes of rats in group 3 were treated with pirenoxine;rats in groups 4,5,6,7,8 and 9 were respectively treated with Dajizhi(Euphorbium)(25 mg/m L),Dajizhi(Euphorbium)(5 mg/m L),Dajizhi(Euphorbium)methanol extract(25 mg/m L),Dajizhi(Euphorbium)methanol extract(5 mg/m L),euphol(25 mg/m L),euphol(5 mg/m L).Cataracts were observed by a slit lamp before and after treatment.Electroretinograms were acquired at set intervals.The morphological changes of the rat eyes were observed in vitro,and the levels of glutathione peroxidase(GSH-Px)and superoxide dismutase(SOD)in the lenses and aqueous humour were estimated at set intervals.RESULTS:Slit lamp examination showed decreased degrees of cataracts after administration of the different treatments.Morphological comparison showed that Dajizhi(Euphorbium)can reduce the turbidity of the lenses,meaning that Dajizhi(Euphorbium)has the anti-cataract effects.Low-concentration of Dajizhi(Euphorbium),its methanol extract and euphol treatment prevented the b-wave amplitudes of the electroretinograms from falling.Euphorbium treatment significantly restored GSH-Px and SOD levels in the lenses and aqueous humour,especially after 10 and 25 d of administration.Euphorbium may help lenses fight oxidative stress caused by selenite.CONCLUSION:The administration of Dajizhi(Euphorbium)can inhibit selenite-induced cataracts.

关 键 词：CATARACT ANTIOXIDANTS ELECTRORETINOGRAM Dajizhi(Euphorbium) 

分 类 号：R285.5[医药卫生—中药学]