马齿苋免煎颗粒对糖尿病性白内障大鼠晶状体整合素β1的影响  

Effect of Portulacae Herba free granuleson integrin-β1 of rats’lens with diabetic cataract

作　　者：刘珺 杨芳 刘江涛 仝警安 周海燕[4] Liu Jun;Yang Fang;Liu Jiangtao;Tong Jing’an;Zhou Haiyan(Department of Ophthalmology,Affiliated Hospital of Shaanxi University of Chinese Medicine,Xianyang 712000,China;Department of Ophthalmology,Nanshi Hospital of Nanyang,Nanyang 473000,China;College of Pharmacy,Shaanxi University of Chinese Medicine,Xianyang 712046,China;Department of Ophthalmology,the People’s Hospital of Shaanxi Provincial,Xi’an 710068,China)

机构地区：[1]陕西中医药大学附属医院眼科,咸阳712000 [2]河南省南阳南石医院眼科,473000 [3]陕西中医药大学药学院,咸阳712046 [4]陕西省人民医院眼科,西安710068

出　　处：《国际中医中药杂志》2021年第12期1215-1218,共4页International Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金(81600720);陕西省自然科学基金(2021JQ739);陕西中医药大学校级课题(2017PY19)。

摘　　要：目的探讨马齿苋免煎颗粒对糖尿病性白内障模型大鼠晶状体混浊程度及整合素β1的影响。方法将50只大鼠按随机数字表法分为对照组、模型组及马齿苋低、中、高剂量组,每组10只。除对照组外,其余各组大鼠腹腔注射1%STZ 60 mg/kg建立糖尿病性白内障大鼠模型。马齿苋低、中、高剂量组灌胃马齿苋免煎颗粒125、250、500 mg/kg,1次/d,连续灌胃12周。裂隙灯下观察各组大鼠晶状体混浊程度,采用HE染色观察晶状体病理学改变,采用Western blot法检测各组大鼠晶状体整合素β1表达。结果与模型组比较,马齿苋低、中、高剂量组大鼠晶状体混浊程度降低(P<0.05),晶状体整合素β1[(1.58±0.14)、(1.46±0.24)、(1.12±0.19)比(1.76±0.23)]表达降低(P<0.05)。结论马齿苋免煎颗粒可降低糖尿病性白内障晶状体整合素β1表达,抑制晶状体混浊。Objective To investigate the effect of Portulacae Herba free granules on the degree of lens opacity and integrinβ1 of lens in diabetic cataract rats.Methods 50 rats were randomly divided into control group,model group and the low,medium and high dose group of Portulacae Herba,10 rats in each group.Except the control group,the diabetic cataract rat model was established by intraperitoneal injection of 1%STZ 60 mg/kg.The low,medium and high dose groups of Portulacae Herba were gavaged with Portulacae Herba 125,250,500 mg/kg,once a day for 12 weeks.The degree of lens opacity was observed with slit lamp.After 12 weeks,the lens was stained with HE.The expression of integrinβ1 was detected by Western blot.Results Compared with the model group,the opacification degree of lens in the low,medium and high dose groups of Portulacae Herba was significantly decreased(P<0.05),the expression of integrinβ1(1.58±0.14,1.46±0.24,1.12±0.19 vs.1.76±0.23)in rat lens was decreased(P<0.05).Conclusion For diabetic cataract rats,Portulacae Herba free granules can influence the expression of integrinβ1 and inhibit the occurance of lens opacity.

关 键 词：马齿苋 糖尿病性白内障 整合素Β1 大鼠 

分 类 号：R285.5[医药卫生—中药学]