基于网络药理学探究苏子降气汤治疗慢性阻塞性肺疾病作用机制  

The mechanism of Suzi-Jiangqi Decoction in treatment of COPD based on network pharmacology

作　　者：康艳 李耀辉[2] 刘伊莎 李哲[3] 张军城 Kang Yan;Li Yaohui;Liu Yisha;Li Zhe;Zhang Juncheng(Pulmonary Disease,Shaanxi Traditional Chinese Medicine Hospitial,Xi’an 710000,China;Department of Traditional Chinese Medicine,Shaanxi University of Traditional Chinese Medicine,Xianyang 712000,China;Chinese Medicine Research and Development Center,China Academy of Chinese Medicine Sciences,Beijing 100700,China)

机构地区：[1]陕西省中医医院肺病科,西安710000 [2]陕西中医药大学中医系,咸阳712000 [3]中国中医科学院中医药发展研究中心,北京100700

出　　处：《国际中医中药杂志》2021年第12期1240-1246,共7页International Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金(81603518);陕西省科技计划厅项目(2020SF-351)。

摘　　要：目的运用网络药理学方法预测苏子降气汤治疗COPD的有效成分和作用靶点,探讨其多成分-多靶点-多通路的潜在分子作用机制。方法依据TCMSP和UniProt软件对苏子降气汤组方中药的有效成分及靶点进行搜集、筛选和预测;在GeneCards、在线人类孟德尔遗传数据库(OMIM)、TTD数据库查询COPD相关基因靶点;使用Veen图在线软件得到中药复方成分靶点与疾病靶点的交集靶点,采用Cytoscape软件构建药物有效成分-靶点-疾病网络并进行分析,使用STRING数据库构建蛋白质相互作用网络(PPI);借助Metascape在线工具对共有靶点进行GO和KEGG通路富集分析。结果筛选出163个苏子降气汤化学成分,预测到283个靶点,涉及COPD的治疗靶点有159个。其中,槲皮素、山柰酚、柚皮素、木犀草素等为关键有效成分。IL6、TNF、MAPK3、JUN、CASP3、CXCL8、CXCL10、MMP-9和MAPK1为重要基因靶点。富集的关键基因靶点涉及的生物学过程包括对细菌的反应、细胞因子介导信号通路、细胞对无机物的反应、对氧化应激的反应和对脂多糖的反应等。富集的关键信号通路包括TNF信号通路、IL-17信号通路、细胞周期、甲型H1N1流感、HTLV-I感染、AGE-RAGE信号通路、结核病和疱疹病毒感染等通路。结论苏子降气汤可通过抗炎、抗感染、调节免疫等多靶点、多通路机制治疗COPD,为其分子机制的研究奠定基础。Objective By using the network pharmacology method to predict the active constituents and action targets of Suzi-Jiangqi Decoction in the treatment of COPD,and to explore its potential molecular mechanism with multi-component,multi-target and multi-pathway characteristics.Methods The active constituents and targets of Suzi-Jiangqi Decoction were collected,screened and predicted according to the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP)and UniProt software.Search for the gene targets related to COPD in GeneCards,online human Mendelian genetic database(OMIM)and TTD database.The intersection targets of component targets and disease targets were obtained by Veen map online software.The network model with the sequence of active constituents-target-disease was constructed and analyzed by Cytoscape software,and the protein-protein interaction network(PPI)was constructed by STRING database.The gene ontology function annotation(GO)and Tokyo genome encyclopedia(KEGG)pathway enrichment analysis of common targets with metascape online tool.Results A total of 163 active constituents of Suzi-Jiangqi Decoction were screened,283 targets were predicted,and 159 targets involved in the treatment of COPD.Quercetin,kaempferol,naringin and luteolin were the key active ingredients.IL6,TNF,MAPK3,JUN,CASP3,CXCL8,CXCL10,MMP9 and MAPK1 were important gene targets.GO analysis showed that the biological processes involved in the enrichment of key gene targets included the response to bacteria,the cytokine mediated signaling pathway,the cell's response to inorganic substances,the response to oxidative stress,the response to LPS,and so on.The enrichment analysis of KEGG pathway showed that the signaling pathway of Suzi-Jiangqi Decoction in the treatment of COPD included TNF signaling pathway,IL-17 signaling pathway,cell cycle,Influenza A,HTLV-I infection,AGE-RAGE signaling pathway,Tuberculosis,Epstein-Barr virus infection and so on.Conclusion Suzi-Jiangqi Decoction can treat COPD through multi-targ

关 键 词：苏子降气汤 肺疾病 慢性阻塞性 有效成分 网络药理学 药理作用分子作用机制(中药) 

分 类 号：R285[医药卫生—中药学]