磷灰石/钛基涂层表面携载高黄芩苷/低栀子苷配伍的协同促内皮抗增殖作用  

The endothelial promotion and anti-proliferation effects of the combination of high baicalin/low geniposide on apatite/titanium-based coating surface

作　　者：杨旭[1] 陈岑[1] 丁亚辉 YANG Xu;CHEN Cen;DING Yahui(College of Life Sciences and Medicine,Zhejiang Sci-TechUniversity,Hangzhou 310018,China;People's Hospital of Hangzhou Medical College,Hangzhou 310014,China;Department of Cardiology,Zhejiang Provincial Peoples Hospital,Hangzhou 310014,China)

机构地区：[1]浙江理工大学生命科学与医药学院,杭州310018 [2]杭州医学院附属人民医院,杭州310014 [3]浙江省人民医院心内科,杭州310014

出　　处：《浙江理工大学学报（自然科学版）》2022年第1期123-130,共8页Journal of Zhejiang Sci-Tech University(Natural Sciences)

基　　金：浙江省中医药管理局计划项目(2018ZB011)。

摘　　要：栀子苷、黄芩苷具有抗血栓、抗炎等药用价值,磷灰石涂层作为药物洗脱支架涂层具有抑制平滑肌细胞(smooth muscle cells, SMCs)增殖能力。为探究磷灰石药物洗脱支架涂层携载黄芩苷/栀子苷对SMCs增殖与内皮细胞(endothelial cells, ECs)生长的作用,将SMCs和ECs分别培养于含50、25 mg/L和12.5 mg/L黄芩苷或栀子苷的培养基中,观察细胞活性,筛选有效作用浓度;在磷灰石涂层表面分别携载10、100μg和150μg栀子苷或黄芩苷,并将细胞接种于涂层表面,观察细胞活性;将150μg黄芩苷/10μg栀子苷配伍、150μg栀子苷/10μg黄芩苷配伍载于磷灰石涂层表面,通过CCK-8、细胞骨架/细胞核染色检测并观察细胞活性及细胞形貌。结果表明:黄芩苷、栀子苷均能在前期抑制SMCs活性并促进ECs增殖。150μg黄芩苷/磷灰石涂层对SMCs的细胞活性有明显的抑制作用,10μg栀子苷/磷灰石涂层相较于100μg和150μg对SMCs的活性影响更大,而对ECs无明显作用;配伍实验显示150μg黄芩苷/10μg栀子苷可显著降低SMCs活性,且对ECs作用较小。因此,高黄芩苷/低栀子苷配伍的磷灰石洗脱涂层有望应用于新一代药物洗脱支架的研究与开发。Geniposide and baicalin have antithrombotic, anti-inflammatory and other medicinal values. Apatite as the coating of drug-eluting stent is able to inhibit the proliferation of smooth muscle cells(SMCs). To explore the effects of drug eluting stent coating with geniposide and baicalin on the proliferation of SMCs and the growth of endothelial cells(ECs), SMCs or ECs were cultured in the medium containing baicalin or geniposide(50, 25 mg/L, and 12.5 mg/L). Then, the cell activity was observed to screen the effective concentration. SMCs and ECs were inoculated on the surface of apatite coating with 10, 100 μg and 150 μg of geniposide or baicalin, respectively, to observe the cell activity. A combination of 150 μg geniposide/10 μg baicalin and 150 μg baicalin/10 μg geniposide were loaded on the surface of apatite coating, respectively. CCK-8 and cytoskeleton/nucleus staining were used to observe the cell activity and morphology. The results showed that both baicalin and geniposide could inhibit SMC proliferation and promote EC growth in the early stage. 150 μg baicalin/apatite coating had a significant inhibitory effect on the cell activity of SMCs, 10 μg geniposide/apatite coating had a greater effect on the cell activity of SMCs compared to 100 μg geniposide/apatite coating and 150 μg geniposide/apatite coating, while no significant effects were shown on ECs. It was found that the combination of 150 μg baicalin/10 μg geniposide significantly decreased SMCs proliferation and had little effect on endothelial cells. Therefore, the combination of high baicalin/low geniposide on apatite coating is expected to be applied to the R & D of new-generation drug-eluting stents.

关 键 词：栀子苷 黄芩苷 磷灰石 配伍载药 平滑肌细胞 内皮细胞 药物洗脱支架 

分 类 号：Q28[生物学—细胞生物学]