Resolving the taxonomy of emerging zoonotic pathogens in the Trichophyton benhamiae complex  

作　　者：AdélaČmoková Miroslav Kolařík Radim Dobiáš Lois L.Hoyer Helena Janouškovcová Rui Kano Ivana Kuklová Pavlína Lysková Lenka Machová Thomas Maier Naďa Mallátová Matěj Man Karel Mencl Pietro Nenoff Andrea Peano Hana Prausová Dirk Stubbe Silke Uhrlaß TomášVětrovský Cornelia Wiegand Vit Hubka 

机构地区：[1]Department of Botany,Faculty of Science,Charles University,Prague,Czech Republic [2]Laboratory of Fungal Genetics and Metabolism,Institute of Microbiology,Czech Academy of Sciences,Prague,Czech Republic [3]Public Health Institute in Ostrava,Ostrava,Czech Republic [4]Department of Biomedical Sciences,Institute of Microbiology and Immunology,Faculty of Medicine,University of Ostrava,Ostrava,Czech Republic [5]Department of Pathobiology,University of Illinois at Urbana-Champaign,Urbana,IL,USA [6]Faculty of Medicine in Pilsen,Biomedical Center,Charles University,Pilsen,Czech Republic [7]Department of Veterinary Dermatology,Nihon University College of Bioresource Sciences,Fujisawa,Japan [8]Department of Dermatology and Venereology,First Faculty of Medicine,General University Hospital in Prague,Charles University and General University Hospital in Prague,Prague,Czech Republic [9]Public Health Institute inÚstíNad Labem,Prague,Czech Republic [10]Microbiological Laboratory/R&D Bioanalytics,Bruker Daltonik GmbH,Bremen,Germany [11]Laboratory of Medical Parasitology and Mycology,HospitalČeskéBudějovice,ČeskéBudějovice,Czech Republic [12]Institute of Botany,Czech Academy of Sciences,Průhonice,Czech Republic [13]Pardubice Regional Hospital,Pardubice,Czech Republic [14]Laboratory of Medical Microbiology,Mölbis,Germany [15]Department of Veterinary Sciences,University of Turin,Turin,Italy [16]Clinical Veterinary Laboratory Labvet,Prague,Czech Republic [17]Mycology and Aerobiology Service,Sciensano,Brussels,Belgium [18]Laboratory of Environmental Microbiology,Institute of Microbiology,Czech Academy of Sciences,Prague,Czech Republic [19]Department of Dermatology,University Hospital Jena,Jena,Germany

出　　处：《Fungal Diversity》2020年第5期I0001-I0055,共55页真菌多样性（英文）

基　　金：Charles University Grant Agency(GAUK 600217):A.Čmoková;Czech Ministry of Health(AZV 17-31269A):M.Kolařík,R.Dobiáš,H.Janouškovcová,I.Kuklová,N.Mallátová,K.Mencl,T.Větrovský,V.Hubka;BIOCEV(CZ.1.05/1.1.00/02.0109)provided;the Ministry of Education,Youth and Sports of the Czech Republic and ERDF:V.Hubka;Charles University Research Centre program no.204069:V.Hubka;Czech Academy of Sciences(Project RVO 67985939):M.Man.

摘　　要：Species of the Trichophyton benhamiae complex are predominantly zoophilic pathogens with a worldwide distribution.These pathogens have recently become important due to their epidemic spread in pets and pet owners.Considerable genetic and phenotypic variability has been revealed in these emerging pathogens,but the species limits and host spectra have not been clearly elucidated.In this study,we used an approach combining phylogenetic analysis based on four loci,population-genetic data,phenotypic and physiological analysis,mating type gene characterization and ecological data to resolve the taxonomy of these pathogens.This approach supported the inclusion of nine taxa in the complex,including three new species and one new variety.Trichophyton benhamiae var.luteum var.nov.(“yellow phenotype”strains)is currently a major cause of zoonotic tinea corporis and capitis in Europe(mostly transmitted from guinea pigs).The isolates of the“white phenotype”do not form a monophyletic group and are segregated into three taxa,T.benhamiae var.benhamiae(mostly North America;dogs),T.europaeum sp.nov.(mostly Europe;guinea pigs),and T.japonicum sp.nov.(predominant in East Asia but also found in Europe;rabbits and guinea pigs).The new species T.africanum sp.nov.is proposed for the“African”race of T.benhamiae.The introduction to new geographic areas and host jump followed by extinction of one mating type gene have played important roles in the evolution of these pathogens.Due to considerable phenotypic similarity of many dermatophytes and phenomena such as incomplete lineage sorting or occasional hybridization and introgression,we demonstrate the need to follow polyphasic approach in species delimitation.Neutrally evolving and noncoding DNA regions showed significantly higher discriminatory power compared to conventional protein-coding loci.Diagnostic options for species identification in practice based on molecular markers,phenotype and MALDI-TOF spectra are presented.A microsatellite typing scheme developed in this study is

关 键 词：Epizootic fungal infections Microsatellite typing scheme Multigene phylogeny Population genetic structure Superficial skin infections Zoophilic dermatophytes 

分 类 号：H31[语言文字—英语]