葛根芩连汤对脂多糖诱导的急性肺损伤大鼠MAPKNF-κB信号通路的调节及肺组织保护作用  

The regulation of Gegen qinlian decoction on MAPK/NF-κB signal pathway and lung tissue protection in rats with acute lung injury induced by lipopolysaccharide

作　　者：刘婷婷[1] 雷华[2] 董红艳 王婷婷[4] 王学武[5] LIU Tingting;LEI Hua;DONG Hongyan;WANG Tingting;WANG Xuewu(Cadre Health Department, The People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi 830001, China;Department of Acupuncture and Massage, The First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, China;Department of Acupuncture, Changji State Hospital of Traditional Chinese Medicine, Changji 831100, Xinjiang,China;Department of Pharmacy, Xinjiang Uygur Autonomous Region People's Hospital, Urumqi 830001,China;Department of Traditional Chinese Medicine, Xinjiang Uygur Autonomous Region People's Hospital, Urumqi 830001, China)

机构地区：[1]新疆维吾尔自治区人民医院干部保健科,新疆乌鲁木齐830001 [2]新疆医科大学第一附属医院针灸推拿科,新疆乌鲁木齐830054 [3]昌吉州中医医院针灸科,新疆昌吉831100 [4]新疆维吾尔自治区人民医院药学部,新疆乌鲁木齐830001 [5]新疆维吾尔自治区人民医院中医科,新疆乌鲁木齐830001

出　　处：《西部医学》2022年第1期40-44,50,共6页Medical Journal of West China

基　　金：新疆维吾尔自治区自然科学基金(2019D01C281)。

摘　　要：目的探讨葛根芩连汤(GQL)对脂多糖诱导的急性肺损伤大鼠MAPK/NF-κB信号通路的调节及肺组织保护作用。方法75只大鼠按照随机数字表法分为5组,分别为对照组、模型组、GQL低、中、高剂量组,15只/组。除对照组外,其余大鼠采用腹腔注射脂多糖(LPS)法建立急性肺损伤大鼠模型,GQL低、中、高剂量组按照2.5 mL/kg的剂量灌胃给予大鼠不同剂量的葛根芩连汤,对照组及模型组灌胃给予大鼠等量生理盐水,1次/d,共给药8周。ELISA法测定各组大鼠血清肿瘤坏死因子-α(TNF-α)、白介素-6(IL-6)及白介素-1β(IL-1β)水平、肺泡灌洗液中性粒细胞(PMN)计数、肺湿干重比值、超氧化物歧化酶(SOD)及肺组织丙二醛(MDA)水平,苏木精-伊红染色法进行大鼠肺组织病理学检查,实时定量PCR检测大鼠肺组织NF-κB/P65及P38/MAPK mRNA水平,Western blot检测大鼠肺组织p-NF-κB/P65、NF-κB/P65、P38/MAPK、p-P38/MAPK蛋白水平。结果与模型组比较,GQL各剂量组大鼠血清TNF-α、IL-6、IL-1β水平、肺泡灌洗液PMN计数、肺组织湿干重比值及MDA水平、肺组织NF-κB/P65及P38/MAPK mRNA水平、p-NF-κB/P65、NF-κB/P65、P38/MAPK、p-P38/MAPK蛋白水平显著降低(均P<0.05),肺组织SOD活性显著升高(P<0.05),大鼠肺组织病理学明显改善。结论葛根芩连汤能够显著降低急性肺损伤大鼠体内炎症因子水平,改善肺组织氧化应激损伤及病理学变化,其机制可能与调节MAPK/NF-κB信号通路有关。Objective To study the regulatory effect of Gegen Qinlian decoction((GQL))on MAPK/NF-κB signal pathway and lung tissue protection in rats with acute lung injury induced by lipopolysaccharide(LPS).Methods 75 rats were randomly divided into control group,model group,low,middle and high dose groups of GQL,15 rats in each group.Except for the control group,the rat model of acute lung injury was established by intraperitoneal injection of lipopolysaccharide((LPS)).The rats in the low,middle and high dose groups of GQL were given different doses of Gegen Qinlian decoction by intragastric administration according to the dose of 2.5mL/kg,once a day for 8 weeks.The levels of tumor necrosis factor-α(TNF--α),interleukin-6(IL-6)and interleukin-1β(IL-1β)in serum,(PMN)count of neutrophils in bronchoalveolar lavage fluid(BALF),wet/dry weight ratio of lung tissue,superoxide dismutase(SOD)activity and malondialdehyde(MDA)level in lung tissue were measured.The lung histopathology of rats was examined by hematoxylin-eosin staining.Real-time quantitative PCR was used to detect the levels of NF-κB/P65 and P38/MAPK mRNA in rat lung tissue.Western blot was used to detect the protein levels of p-NF-κB/P65,NF-κB/P65,P38/MAPK and p-P38/MAPK in rat lung tissue.Results Compared with the model group,the levels of TNF-α,IL-6 and IL-1βin serum,(PMN)count of neutrophils in bronchoalveolar lavage fluid,wet/dry weight ratio and MDA level of lung tissue,NF-κB/P65 and P38/MAPK mRNA levels in lung tissue,protein levels of p-NF-κB/P65,NF-κB/P65,P38/MAPK and p-P38/MAPK in lung tissue of rats in each dose group were significantly lower than those in model group(P<0.05),while SOD activity in lung tissue was significantly increased(P<0.05).The lung histopathology of rats was significantly improved.Conclusion Gegen Qinlian decoction can significantly reduce the level of inflammatory factors and improve the oxidative stress injury and pathological changes of lung tissue in rats with acute lung injury,and its mechanism may be related to the reg

关 键 词：根芩连汤 急性肺损伤 MAPK/NF-κB信号通路 肺组织保护作用 

分 类 号：R563[医药卫生—呼吸系统]