NaCl胁迫对单叶蔓荆幼苗矿质元素分布及部分生理指标的影响  

Effects of NaCl stress on the compartmental allocation of mineral element and some physiological characteristics of Vitex rotundifolia seedlings

作　　者：卞阿娜 陆銮眉 BIAN Ana;LU Luanmei(Department of Biological Science and Biotechnology,Minnan Normal University,Key Laboratory of Landscape Plants in Fujian and Taiwan Characteristics of Fujian Colleges and Universities,Zhangzhou,Fujian 363000,China)

机构地区：[1]闽南师范大学生物科学与技术学院,闽台特色园林植物福建省高校重点实验室,福建漳州363000

出　　处：《植物生理学报》2021年第12期2291-2300,共10页Plant Physiology Journal

基　　金：福建省科技重点项目(2017N01010156);漳州市科技重点项目(ZZ2014036);闽南师范大学创新团队(2016)。

摘　　要：在温室培养条件下,对盐生植物单叶蔓荆(Vitex rotundifolia)的幼苗进行NaCl溶液胁迫(0、5、10、15、20和25 g·L^(-1))处理,研究NaCl胁迫下单叶蔓荆叶片无机离子(Na^(+)、Cl^(-)、K^(+)、Mg^(2+)、Ca^(2+))含量及部分生理指标的变化,以探讨单叶蔓荆响应NaCl胁迫的耐盐适应机制。结果表明:随NaCl浓度的升高单叶蔓荆幼苗生长(高度和叶片数)下降,叶片灰分含量呈先升后降趋势;NaCl胁迫单叶蔓荆幼苗的叶片坏死斑主要分布于叶片中心区和老叶;幼苗叶片的Na^(+)和Cl^(-)含量显著增加且存在极显著强正相关,离子毒害主要由Na^(+)和Cl^(-)共同造成;NaCl胁迫下单叶蔓荆幼苗叶片K^(+)/Na^(+)、Mg^(2+)/Na^(+)和Ca^(2+)/Na^(+)值均低于对照组,且随盐度递增呈先降后升趋势;单叶蔓荆幼苗可溶性糖和脯氨酸含量随盐度递增而递增, MDA含量逐渐下降,SOD活性和POD活性呈先升后降趋势;低盐胁迫下单叶蔓荆幼苗能够迅速合成较多的SOD和POD保护酶来抵抗NaCl胁迫,减少对膜系统的伤害,保持一定的耐盐性;单叶蔓荆适生的生长耐受临界NaCl浓度为10g·L^(-1),在滨海区绿化上具有良好的应用前景。To understand the salt-tolerant mechanism of halophyte Vitex rotundifolia seedlings, the seedlings were subjected to a substrate salinity gradient(0, 5, 10, 15, 20 and 25 g·L^(-1)) in the greenhouse, and the changes of mineral elements(Ca^(2+), Mg^(2+), Na^(+), K^(+)and Cl^(-)) and some physiological indexes were measured and analyzed. The results showed that the growth(height and leaf number) of seedlings decreased,and the content of ash increased first and then decreased with the increase of salinity. The leaf necrotic spots were mainly distributed in the center part of leaves and the old leaves under substrate salt stress.The contents of Na^(+)and Cl^(-)in seedlings increased significantly under NaCl stress, and there was a very significant positive correlation. The ion toxicity was mainly caused by the combination of Na^(+)and Cl^(-). The ratios of K^(+)/Na^(+), Mg^(2+)/Na^(+)and Ca^(2+)/Na^(+)in V. rotundifolia seedlings were lower than those in the control group under NaCl stress, which decreased first and then increased with the increase of salinity. The contents of soluble sugar and proline in leaves of V. rotundifolia increased gradually, while the content of MDA decreased gradually, and the activities of SOD and POD increased firstly and then fell along with the increase of salinity. Under low salinity stress, more protective enzymes(SOD and POD) can be rapidly synthesized to reduce membrane system damage. The critical salt concentration of V. rotundifolia seedlings was 10 g·L^(-1), which has a good application prospect in coastal greening.

关 键 词：NACL胁迫 单叶蔓荆 离子分布 生理特性 

分 类 号：S793.9[农业科学—林木遗传育种]