武汉儿童医院就诊儿童群体乙型肝炎病毒血清标志物检测分析  

Detection and analysis of hepatitis B virus serum markers in children treated in Wuhan Children′s Hospital

作　　者：黄永国 孙红[1] 向贇[1] HUANG Yong-guo;SUN Hong;XIANG Yun(Department of Laboratory Medicine,Wuhan Children′s Hospital,Tongji Medical College,Huazhong University of Science&Technology,Wuhan 430016,China)

机构地区：[1]华中科技大学同济医学院附属武汉儿童医院检验部,武汉430016

出　　处：《公共卫生与预防医学》2022年第1期66-70,共5页Journal of Public Health and Preventive Medicine

基　　金：武汉市卫生健康委科研计划资助项目(WX15D37)。

摘　　要：目的了解武汉地区儿童群体乙型肝炎病毒(hepatitis B virus,HBV)感染现状,分析其血清标志物表达模式及分布特征。方法采用电化学发光免疫分析技术对武汉儿童医院住院、门诊以及体检的67027例0d~18岁儿童HBsA、HBsAb、HBeAg、HBeAb、HBcAb五项血清标志物进行检测,使用SPSS 24.0统计软件对检测结果分年龄段和性别进行统计分析。结果所有67027名儿童"全阴性"检出率为18.98%,HBcAb阳性率男女比较差异有统计学意义;0~28d及1~12月组有较高的HBcAb阳性率,1岁以后显著降低,1~14岁HBcAb阳性率为5.02%,而15~18岁略有升高。HBsAb阳性儿童中,1~2岁组HBsAb阳性率达峰值95.65%,6~14岁组最低68.90%,至15岁以前呈逐渐下降趋势。HBsAb浓度≥100 IU/L儿童中,以1~2岁组人数占比最高(76.99%),6~14岁组占比最低(40.99%)。共检出HBV血清标志物表达模式20种,占比≥1.00%的分别为"单HBsAb+","全阴性","HBsAb+/HBcAb+","HBsAb+/HBeAb+/HBcAb+"检出率分别为71.40%、18.98%、4.80%和4.20%。其中不常见表达模式11种,新生儿组检出不常见模式9种,检出率为1.21%,高于其他各年龄组。所有血清学模式中,仅"单项HBcAb+"检出率不同性别之间比较差异有统计学意义。结论武汉地区0~18岁儿童群体各年龄段HBV感染率较低;"单HBsAb+"为主要的血清学模式,HBsAb浓度分布多位于100~999IU/L,同时存在较高的"全阴性"检出率,学龄期儿童应补种乙肝疫苗,可能有利于降低感染风险。Objective To investigate the status of hepatitis B virus(HBV)infection in children in Wuhan,and to analyze the expression pattern and distribution of serum markers.Methods Five serum markers of HBsA,HBsAb,HBeAg,HBeAb and HBcAb were detected by electrochemiluminescence immunoassay in 67027 children aged 0-18 years including inpatients,outpatients,and physical examinees in Wuhan Children′s Hospital.SPSS 24.0 statistical software was used to analyze the results by age and gender.Results The"all negative"detection rate of all 67027 children was 18.98%.There was a significant difference in the positive rate of HBcAb between male and female.The positive rate of HBcAb was higher in 0~28 days and 1~12 months group and decreased significantly after 1 year old.The positive rate of HBcAb was 5.02%in 1-14 years old but increased slightly in 15-18 years old.Among HBsAb positive children,the positive rate of HBsAb reached the peak of 95.65%in 1~2 years old group and the lowest of 68.90%in 6~14 years old group,and gradually decreased before 15 years old.Among the children with HBsAb concentration≥100 IU/L,the proportion of 1~2 years old group was the highest(76.99%),and the proportion of 6~14 years old group was the lowest(40.99%).A total of 20 HBsAb serum marker expression patterns were detected,and the detection rates of"single HBsAb+","all negative","HBsAb+/HBcAb+",and"HBsAb+/HBeAb+/HBcAb+"were 71.40%,18.98%,4.80%and 4.20%,respectively.Among them,11 kinds of uncommon expression patterns were detected,and 9 kinds of uncommon expression patterns were detected in neonates,with a detection rate of 1.21%,which was higher than that in other age groups.Among all serological patterns,only the detection rate of"single HBcAb+"showed a statistical difference between male and female.Conclusion The HBV infection rate in all ages of 0~18 years old children in Wuhan is low."Single HBsAb+"is the main serological pattern,and the concentration distribution of HBsAb is mostly in the range of 100-999 IU/L.There is a high"all negative"detect

关 键 词：儿童 乙型肝炎病毒 血清学标志物 电化学发光免疫法 

分 类 号：R181[医药卫生—流行病学]