老年肺腺癌胸腔积液沉淀物免疫组化检测技术的临床诊断应用  

Clinical diagnostic usage of pleural effusion immunohistochemical staining in elderly lung adenocarcinoma

作　　者：舒守宏[1] 朱彦 陈久红 邢益祥 SHU Shou-hong;ZHU Yan;CHEN Jiu-hong;XING Yi-xiang(Department of General Practice Medicine,Tongling People's Hospital,Tongling 244000,Anhui Province,China)

机构地区：[1]铜陵市人民医院全科医学科,安徽铜陵244000

出　　处：《中国临床药理学杂志》2021年第24期3315-3318,共4页The Chinese Journal of Clinical Pharmacology

基　　金：铜陵市卫生和计划生育委员会医学科研基金资助项目(卫科研[2016]23号)。

摘　　要：目的探索胸腔积液沉淀物免疫组化检测技术在老年患者肺腺癌临床诊断、判断转移的作用。方法选取30例原发肺腺癌伴胸腔积液的老年患者为试验组,30例良性胸腔积液老年患者为对照组。分析细胞角蛋白7(CK7)、甲状腺转录因子-1(TTF1)、新天冬氨酸蛋白酶A(Napsin A)、钙调蛋白(CR)、B细胞特性莫洛尼鼠白血病病毒插入位点1(Bmi-1)在胸腔积液沉淀物及肺组织中的表达水平及其与临床特征的关系。结果CK7、TTF1、Napsin A和Bmi-1在试验组中的高表达率分别为100.0%,86.7%,93.3%和83.3%,而在对照组中分别为13.3%,10.0%,10.0%和6.7%,差异均有统计学意义(均P<0.001)。各指标蛋白表达与临床病理特征均无显著相关性。结论胸腔积液沉淀物细胞蜡块免疫组化检测(以Bmi-1蛋白表达为补充检测手段)可替代传统实体病理切片,用作临床诊断老年肺腺癌的重要参考指标,有耐受性好、安全性佳等优点。Objective To explore the usage of immunohistochemical staining of pleural effusion sediments in the clinical diagnosis and the judgment of metastasis of elderly patients with lung adenocarcinoma. Methods Thirty elderly patients with primary lung adenocarcinoma and pleural effusion were selected as the treatment group, and 30 elderly patients with benign pleural effusion were selected as benign controls group. The expression level of cytokeratin 7(CK7), thyroid transcription factor 1(TTF1), novel aspartic proteinase of the pepsin family A(Napsin A), calretinin(CR), B-cell-specific Moloney murine leukemia virus insertion site 1(Bmi-1) in pleural effusions and lung tissues, and their relationships with clinical case characteristics were analyzed. Results In the treatment group, the high expression rates of CK7, TTF1, Napsin A and Bmi-1 in lung adenocarcinoma pleural effusion and lung adenocarcinoma tumor tissue had reached 100.0%, 86.7%, 93.3% and 83.3%, while those in the control group were 13.3%, 10.0%, 10.0% and 6.7%, showing extremely obvious differences(all P<0.001). No significant relationships were found between marker proteins expression and clinical case characteristics. Conclusion Immunohistochemistry staining in pleural effusion sediment cell wax blocks(using Bmi-1 expression level as a supplement) could replace traditional solid tumor pathological section,which can be employed for judging the metastasis and the prognosis of lung adenocarcinoma with better tolerance and less risks.

关 键 词：肺腺癌 胸腔积液 B细胞特性莫洛尼鼠白血病病毒插入位点1 

分 类 号：R97[医药卫生—药品]