利用转录组测序筛选鸡繁殖性状相关lncRNAs  

Screening of LncRNAs Related to Chicken Reproductive Traits by Using RNA-seq

作　　者：李春苗[1,2] 贾雪波 黄正洋[1,2] 黎寿丰[1,2] 黄华云 王钱保[1,2] 薛龙岗 赵振华[1,2] LI Chunmiao;JIA Xuebo;HUANG Zhengyang;LI Shoufeng;HUANG Huayun;WANG Qianbao;XUE Longgang;ZHAO Zhenhua(Jiangsu Institute of Poultry Sciences,Yangzhou,Jiangsu 225125;Key Laboratory for Poultry Genetics and Breeding of Jiangsu Province,Yangzhou,Jiangsu 225125)

机构地区：[1]江苏省家禽科学研究所,江苏扬州225125 [2]江苏省家禽遗传育种重点实验室,江苏扬州225125

出　　处：《中国家禽》2021年第12期7-13,共7页China Poultry

基　　金：现代农业产业技术体系建设专项资金(CARS-41-Z05);江苏省农业科技自主创新项目(CX(20)2012);江苏省科技成果转化项目(BA2019049);江苏省科技成果转化项目子课题(BA201904901);江苏省公益类科研院所自主科研经费项目(BM2018026-2);江苏省家禽遗传育种重点实验室资助项目(JQLAB-ZZ-202008)。

摘　　要：为探讨长链非编码RNA(Long non-coding RNA,lncRNA)在鸡繁殖性状中的作用,研究通过分析苏禽3号高产组和低产组母鸡垂体转录组数据,结合edgeR软件预测出在鸡垂体中差异表达的lncRNA,并通过GO和KEGG pathway富集分析,找出与鸡繁殖性状相关的差异表达lncRNA。结果表明,通过预测得到952个差异表达的lncRNA,其中908个上调,44个下调;差异表达的lncRNA共富集到9个信号通路,与繁殖性状相关的通路分别为BMP和cAMP,共筛选到3个与繁殖性状相关的lncRNA,推测这3个lncRNA在鸡繁殖过程中起重要作用,但其机制还需要进一步研究。研究结果对进一步深入研究与鸡产蛋性能相关的lncRNA生物学功能及调控机制奠定了基础,丰富了鸡的生殖调控理论。The experiment was conducted to study the lncRNA function in reproductive traits in chicken.In this study,lncRNA candidates were identified combining edgeR software and analysed the sequence high-throughput RNA-seq based on the chicken pituitary transcriptome data from Suqin No.3 high-yield and low-yield hens.Through GO and KEGG pathway enrichment analysis,the differential expression of lncRNAs related to reproductive traits were found out.The results showed that 952 differentially expressed lncRNAs were obtained through prediction,that 908 were positive accommodation and 44 were negative regulation.The differential expressed lncRNAs were enriched in 9 signal pathways.The pathways related to reproductive traits were BMP and cAMP,and 3 lncRNAs related to reproductive traits were screened.It was speculated that these 3 lncRNAs played an important role in the reproductive process of chickens,but their mechanisms needed further to study.This result laid a foundation for further in-depth study on the biological function and regulation mechanism of lncRNA related to chicken laying performance,and enriched the theory of chicken reproduction regulation.

关 键 词：转录组 鸡 垂体 lncRNA 

分 类 号：S831.2[农业科学—畜牧学]