长链非编码RNA牛磺酸上调基因在肝癌组织中表达及对肝癌细胞活性的影响  

Expression of Long Noncoding RNA in Hepatocellular Carcinoma and Its Effect on Activity of Hepatocellular Carcinoma Cells

作　　者：邵旭辉[1] 丁希艳[1] 李彦冬[1] SHAO Xuhui;DING Xiyan;LI Yandong(Affiliated Hospital of Beihua University,Jilin 132011,China)

机构地区：[1]北华大学附属医院,吉林吉林132011

出　　处：《北华大学学报（自然科学版）》2021年第6期748-752,共5页Journal of Beihua University（Natural Science）

基　　金：吉林省卫生与健康技术创新项目(2019J039);吉林省教育厅科学技术项目(JJKH20200065KJ);吉林市医疗卫生指导性计划项目(2021001345).

摘　　要：目的探讨长链非编码RNA牛磺酸上调基因(lncRNA TUG1)在肝癌组织中的表达及对肝癌细胞活性的影响.方法收集经手术治疗及肝脏穿刺确诊的肝细胞癌患者标本,取其对应的肿瘤组织、肿瘤边缘组织以及周边组织(无癌细胞组织)标本,另外一部分标本来源于既往实验剩余标本,共126例.应用原位杂交法检测lncRNA TUG1表达情况,然后体外培养HCCLM3细胞,并将其分为3组:空白对照组(常规培养)、si-NC组(空载体质粒)、si-TUG1组(lncRNA TUG1表达抑制载体质粒).qRT-PCR检测细胞中lncRNA TUG1的表达;Transwell实验检测细胞的迁移和侵袭能力;Western blot检测细胞中磷脂酰肌醇-3激酶、蛋白激酶B蛋白表达.结果lncRNA TUG1在肝癌组织中的表达量明显高于癌旁正常组织,差异具有统计学意义(P<0.01);肝癌组织中lncRNA TUG1的高表达率在不同年龄、肿瘤直径、TNM分期、淋巴结转移情况、肿瘤分化程度的肝癌患者之间比较差异具有统计学意义(P<0.01).si-TUG1组肝癌细胞lncRNA TUG1表达量明显低于si-NC组、空白对照组,差异具有统计学意义(P<0.01).si-TUG1组迁移细胞数和侵袭细胞数明显高于空白对照组、si-NC组,差异具有统计学意义(P<0.01).si-TUG1组肝癌细胞中磷脂酰肌醇-3激酶、蛋白激酶B的表达量明显低于空白对照组、si-NC组,差异具有统计学意义(P<0.05).结论lncRNA TUG1在肝癌组织中高表达;下调lncRNA TUG1表达能够降低肝癌细胞的迁移和侵袭能力,其机制与抑制磷脂酰肌醇-3激酶/蛋白激酶B通路活性密切相关.Objective To investigate the expression of taurine upregulated gene(TUG1)of long noncoding RNA(lncRNA)in hepatocellular carcinoma(HCC)and its effect on the activity of HCC cells.Method Collect specimens from surgical treatment and liver biopsy diagnosis of hepatocellular carcinoma patients,and take the corresponding edge of tumor tissue,tumor and surrounding tissue(not cancerous tissue)specimens,another part of the specimen is derived from the previous experimental specimens,remaining a total of 126 cases,in situ hybridization detection TUG1 expression.HCCLM3 cells were cultured in vitro and divided into three groups:blank control group(conventional culture),si-NC group(empty particles),si-TUG1 group(lncRNA TUG1 expression inhibition vector plasmid).The expression of lncRNA TUG1 was detected by qRT-PCR;Cell migration and invasion was used to detect Transwell;Western blot was used to detect the protein expression of phosphatidylinositol-3 kinase and protein kinase B.Results The expression of lncRNA TUG1 in HCC tissues was significantly higher(P<0.01);There were significant differences in the high expression rate of lncRNA TUG1 among lung cancer patients with different age,tumor size,TNM stage,lymph node metastasis and tumor differentiation(P<0.01).The expression of lncRNA TUG1 in si-TUG1 group was significantly lower than that in si-NC group and blank control group(P<0.01).The number of migrating cells and invasive cells in si-TUG1 group was significantly higher(P<0.01).The expression levels of phosphatidylinositol-3 kinase and protein kinase B in si-TUG1 group were significantly lower(P<0.05).Conclusion lncRNA TUG1 is highly expressed in HCC;Down-regulation of lncRNA TUG1 expression can reduce the migration and invasion ability of hepatoma cells,and its mechanism is related to the inhibition of phosphatidylinositol-3 kinase/protein kinase B pathway activity.

关 键 词：肝癌 长链非编码RNA 牛磺酸上调基因 肝癌细胞活性 

分 类 号：R605[医药卫生—外科学]