HIV/AIDS合并CM患者IL-10对Th1/Th2免疫调节机制探讨  

Study on the immunoregulation mechanism of IL-10 on Th1/Th2 in HIV/AIDS patients with CM

作　　者：磨立达 黄宛虹 刘泽端 罗晓璐 MO Lida;HUANG Wanhong;LIU Zeduan;LUO Xiaolu(Department of Clinical Laboratory,Nanning Fourth People's Hospital/Nanning Infectious Disease Hospital Affiliated to Guangxi Medical University/Guangxi AIDS Clinical Treatment Center(Nanning),Nanning,Guangxi 530023,China)

机构地区：[1]南宁市第四人民医院/广西医科大学附属南宁市传染病医院/广西艾滋病临床治疗中心(南宁)检验科,广西南宁530023

出　　处：《检验医学与临床》2022年第1期26-30,共5页Laboratory Medicine and Clinic

基　　金：广西壮族自治区卫生和计划生育委员会自筹经费科研课题(Z2016067);广西壮族自治区南宁市科学技术局重大专项(20193008)。

摘　　要：目的分析人类免疫缺陷病毒(HIV)/艾滋病(AIDS)合并新型隐球菌脑膜炎(CM)患者白细胞介素(IL)-10与常见细胞因子的相关性,探讨其对辅助性T细胞(Th)1/Th2免疫调节机制。方法选择40例HIV/AIDS合并CM患者为A组,40例HIV/AIDS合并结核分枝杆菌感染患者为B组,40例单纯HIV/AIDS感染患者为C组,40例CM患者为D组。对每组患者进行血浆和脑脊液IL-4、IL-6、IL-10、IL-12、干扰素-γ(IFN-γ)及肿瘤坏死因子-α(TNF-α)水平检测,分析IL-10与其他因子的相关性。结果 A组血浆及脑脊液IL-10水平与其他3组比较,差异均无统计学意义(P>0.05)。经Pearson相关分析,A组血浆中,IL-10与IL-4、IL-6、IL-12的r值分别为0.898、0.866、0.729,P<0.001;A组脑脊液中,IL-10与IL-4、TNF-α的r值分别为0.852、0.714,P<0.05。经校正,对于A组,血浆IL-10低值的患者IFN-γ水平高于IL-10高值的患者,IL-10低值的患者IL-4、IL-6、IL-12水平低于IL-10高值的患者,差异均有统计学意义(P<0.05);对于A组,脑脊液IL-10低值的患者IL-4水平低于IL-10高值的患者,差异有统计学意义(P<0.05)。结论 IL-10在HIV/AIDS合并CM持续感染中,既可发挥负调节作用,又可发挥免疫抑制作用,能为诊治提供依据。Objective To analyze the correlation between interleukin-10(IL-10) and common cytokines in human immunodeficiency virus(HIV)/acquired immune deficiency syndrome(AIDS) patients with cryptococcus neoformans meningitis(CM),explore the mechanism of helper T cell(Th)1/Th2 immunoregulation.Methods 40 HIV/AIDS patients with CM were selected as group A,40 cases of HIV/AIDS patients with tuberculosis infection as group B,40 patients with simple HIV/AIDS infection were selected as group C,40 patients with CM were divided into Group D.The levels of IL-4,IL-6,IL-10,IL-12 and interferon in plasma and cerebrospinal fluid were measured in each group-γ(IFN-γ) Tumor necrosis factor-α(TNF-α) The correlation between IL-10 and other factors was analyzed.Results There was no significant difference in IL-10 in plasma and cerebrospinal fluid between group A and the other three groups(P>0.05).Pearson correlation analysis showed that the r values of IL-10,IL-4,IL-6 and IL-12 in group A were 0.898,0.866 and 0.729,respectively, P<0.001.The r value of IL-10 and IL-4 in CSF of group A was 0.852,IL-10 and TNF-α in CSF of group A was 0.741,P<0.05.After correction, for group A,the levels of IFN-γ in plasma low IL-10 were significantly higher than those in high IL-10,and the levels of IL-4,IL-6 and IL-12 in low IL-10 were significantly lower than those in high IL-10,the difference was statistically significant(P<0.05).For group A,the level of IL-4 in CSF low IL-10 was significantly lower than that in CSF high IL-10,the difference was statistically significant(P<0.05).Conclusion IL-10 can not only play a negative regulatory role in HIV/AIDS complicated with persistent CM infection, but also play an immunosuppressive role, which can provide evidence for diagnosis and treatment.

关 键 词：免疫调节机制 白细胞介素-10 新型隐球菌脑膜炎 人类免疫缺陷病毒 艾滋病 

分 类 号：R759.1[医药卫生—皮肤病学与性病学]