川黄柏中原小檗碱型生物碱及其细胞毒性研究  

Berberine-type Alkaloids from Phellodendron Chinense and Their Anti-tumor Activities

作　　者：闫玉鑫[1] 杨颖 池利昆[1] 郑萍[1] YAN Yu-xin;YANG Ying;CHI Li-kun;ZHENG Ping(College of Vocational and Technical Education,Yunnan Normal University,Kunming 650092,China)

机构地区：[1]云南师范大学职业技术教育学院,云南昆明650092

出　　处：《云南师范大学学报（自然科学版）》2022年第1期46-49,共4页Journal of Yunnan Normal University:Natural Sciences Edition

基　　金：国家自然科学基金资助项目(82003616);云南省教育厅科学研究基金资助项目(2014Y146).

摘　　要：采用甲醇溶剂对芸香科黄柏属川黄柏的茎进行回流提取,利用硅胶、胺基硅胶和葡聚糖凝胶等多种柱色谱法对提取物化学成分进行研究,通过核磁共振和电喷雾质谱等波谱技术以及与文献报道数据比对,从中分离鉴定了4个原小檗碱型生物碱,分别鉴定为二氢小檗碱(1),hediamine(2),2-methoxy-berbin-10-ol(3),3-methoxy-berbin-10-ol(4).并对化合物1的波谱进行详细归属,对化合物2进行单晶衍射实验,确定其绝对构型.所有化合物均对人肝癌细胞(HepG2)有较强的细胞毒性.Various techniques were used to study the chemical constituents and the anti-tumor effect of Phellodendron chinense.Four compounds were isolated and identified as as pachycanthine(1),hediamine(2),2-methoxy-berbin-10-ol(3),3-methoxy-berbin-10-ol(4).The spectrum of compound 1 was assigned in detail,and the X-ray crystal diffraction experiment of compound 2 was performed.All compounds have strong cytotoxicity to HepG2 cell.

关 键 词：黄柏 小檗碱 二氢小檗碱 hediamine 2-methoxy-berbin-10-ol 3-methoxy-berbin-10-ol 

分 类 号：R284[医药卫生—中药学]