花花柴抗逆相关基因KcCIPK9的克隆及原核表达  

Cloning and prokaryotic expression of the stress resistance related gene KcCIPK9 from Karelinia capsica

作　　者：陆忻子墨 黄海涛 孔繁雨 李小龙 张晓楠 吴莹莹 杨静 王彦芹 LU Xinzimo;HUANG Haitao;KONG Fanyu;LI Xiaolong;ZHANG Xiaonan;WU Yingying;YANG Jing;WANG Yanqin(College of Life Sciences,Tarim University/Xinjiang Production&Construction Corps Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin.Alar,Xinjiang 843300)

机构地区：[1]塔里木大学生命科学学院/塔里木盆地生物资源保护利用兵团重点实验室,新疆阿拉尔843300

出　　处：《塔里木大学学报》2021年第4期1-7,共7页Journal of Tarim University

基　　金：国家自然科学基金项目“沙漠植物花花柴花器官耐高温的生物学功能分析”(31660085)。

摘　　要：荒漠植物花花柴具有极强的抗非生物胁迫能力,CBL-CIPK通路是其所具有的较强的非生物胁迫能力重要来源之一。本试验克隆花花柴KcCIPK9基因的cDNA全长,构建原核表达载体并在大肠杆菌(E.coli)中超表达,通过对E.coli分别在高温胁迫、聚乙二醇-4000(PEG-4000)渗透胁迫条件下的繁殖速率,分析花花柴KcCIPK9基因的抗逆性。结果表明:花花柴KcCIPK9的cDNA全长是1395 bp,编码464个氨基酸;含有KcCIPK9重组质粒的E.coli相比不含有KcCIPK9重组质粒的E.coli具有更强的耐高温性和耐旱性。表明荒漠植物花花柴的KcCIPK9基因可以在大肠杆菌中原核表达,且具有增强宿主菌抗逆性的能力。Desert plant Karelinia capsica has strong ability to resist abiotic stress.CBL-CIPK pathway is one of the important sources of its strong abiotic stress ability.In this experiment,the full-length cDNA of KcCIPK9 gene was cloned,the prokaryotic ex-pression vector was constructed and overexpressed in Esherichia coli(E.coli).The stress resistance of KcCIPK9 gene was analyzed under high temperature stress and polyethylene glycol-4000(PEG-4000)osmotic stress.The results showed that the cDNA full length of KcCIPK9 was 1395 bp,encoding 464 amino acids.The stress resistance to high temperature and drought is higher than wild type E.coli.The results showed that KcCIPK9 gene could express in prokaryotic and had the ability to enhance the stress resis-tance of host bacteria.

关 键 词：花花柴 CIPK9 原核表达 非生物胁迫 

分 类 号：Q78[生物学—分子生物学]