基于BSA-seq的黄褐棉纤维长度候选基因发掘  

Exploration of Candidate Genes for Fiber Length in Gossypium mustelinum Based on BSA-seq

作　　者：陈奇 王议萍 陈艳 周婉婷 汤备 朱心慧 于梓璇 李馨蕊 汪保华[1] CHEN Qi;WANG Yiping;CHEN Yan;ZHOU Wanting;TANG Bei;ZHU Xinhui;YU Zixuan;LI Xinrui;WANG Baohua(School of Life Sciences,Nantong University,Nantong 226019,China)

机构地区：[1]南通大学生命科学学院,江苏南通226019

出　　处：《南通大学学报（自然科学版）》2021年第4期58-64,94,共8页Journal of Nantong University(Natural Science Edition)　

基　　金：国家重点研发计划项目(2021YFE0101200);江苏省研究生科研创新计划项目(KYCX19_2048);国家级大学生创新创业训练计划项目(201910304054Z)。

摘　　要：纤维长度是棉花纤维品质的重要性状之一,发掘纤维长度基因对选育优质棉花品种具有重要意义。前期研究基于一套黄褐棉导入系群体定位到15个纤维长度数量性状基因座(quantitative trait locus,QTL),并分子辅助选育出一个纤维品质表现突出的黄褐棉导入系IL9,与其陆地棉轮回亲本PD94042杂交并自交构建了F2大群体。基于该群体的表型数据构建了纤维长度最长和最短的两个DNA混池,连同两个亲本共4个混池,利用极端性状混池重测序(bulked-segregant analysis sequencing,BSA-seq)方法进行遗传关联性分析,共检测到34个控制纤维长度的潜在候选基因,分别定位在Chr.7、Chr.10、Chr.18共3条染色体上的3个相关区域内。通过比较QTL定位、BSA-seq分析的结果,以及相关功能注释筛选,最终得到3个候选基因(Gh_D13G1294,Gh_D13G1295,Gh_D13G1296),这些候选基因可能对改良陆地棉纤维长度起重要作用。Fiber length is one of the important characteristics of cotton fiber quality.It is of great significance to explore fiber length genes for breeding of cotton varieties with elite fiber quality.In the first phase of the study,15 quantitative trait loci(QTLs)of fiber length were mapped based on a set of Gossypium mustelinum introgression lines,and an introgression line IL9 with elite fiber quality was selected by molecular marker-assisted breeding.A large F2 population was constructed by crossing of IL9 with the recurrent Upland cotton parent PD94042.Four DNA pools,namely DNA pools with the longest fiber length,the shortest fiber length,and the two parents,were subjected to next-generation sequencing-based bulked-segregant analysis(BSA-seq).Totally 34 potential candidate genes controlling fiber length were located in three regions on Chr.7,Chr.10,and Chr.18.By comparing the results of QTL mapping and BSA-seq,three candidate genes,namely Gh_D13G1294,Gh_D13G1295,and Gh_D13G1296 were obtained by functional annotation.These genes may play important roles in the improvement of fiber length in Upland cotton.

关 键 词：黄褐棉 纤维长度 分子育种 极端性状混池重测序 候选基因 

分 类 号：Q37[生物学—遗传学]