反复冷-热刺激对大鼠鼻黏膜免疫屏障功能的影响及中医解表方的作用研究  

Effect of the superficies-resolving formula of traditional Chinese medicine on cold-warm cycle stimulation and nasal mucosal immune barrier function in rats

作　　者：杨居崩 赵显芳 聂发龙 肖纯 代蓉 李秀芳 YANG Jubeng;ZHAO Xianfang;NIE Falong;XIAO Chun;DAI Rong;LI Xiufang(Yunnan University of Chinese Medicine,Kunming 650500,China)

机构地区：[1]云南中医药大学,昆明650500

出　　处：《中国实验动物学报》2021年第6期715-723,共9页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家自然科学基金项目(81860724);云南省科技厅-中医联合专项项目(202001AZ070001-001,2019FF002(-054))。

摘　　要：目的探讨反复冷-热刺激对大鼠鼻黏膜免疫屏障功能的影响,考察中医常用解表方麻黄汤和银翘散的作用。方法将SD大鼠置于-15℃1 h,再转入25℃30 min,冷-热交替刺激4次,末次刺激后检测不同时间点鼻黏膜中IgA蛋白表达水平,检测1 d后鼻腔灌洗液中溶菌酶、sIgA及PGE2含量,血清中的炎症因子含量;冷-热刺激后第9天,再次给予相同条件刺激,分别于末次刺激后1~11 d检测鼻黏膜中IgA蛋白动态变化,鼻腔灌洗液中溶菌酶、PGE2动态变化,血清中炎症因子动态变化,再次刺激1 d后鼻腔灌洗液中sIgA含量;解表方麻黄汤2.43 g生药/kg BW水煎液、银翘散33.48 g生药/kg BW水煎液对两轮冷-热刺激的大鼠进行干预,经第二轮冷-热刺激24 h后检测相应指标。结果反复冷-热刺激后,大鼠鼻黏膜IgA蛋白表达在刺激后1、3 d明显降低,随后逐渐上升,刺激后7 d恢复正常;刺激后1 d sIgA明显降低,溶菌酶及炎症因子无明显变化。当大鼠再次受到冷-热刺激后,鼻黏膜IgA蛋白表达显著下降,鼻腔灌洗液中溶菌酶及PGE2的含量明显降低;血清中TNF-α、PGE2、IL-1β及IL-6的含量显著升高。麻黄汤和银翘散干预后,两方均可升高大鼠鼻黏膜中IgA蛋白表达及鼻腔灌洗液中sIgA的含量,银翘散还可升高溶菌酶含量,降低血清的TNF-α、IL-1β含量。结论反复冷-热刺激可导致大鼠鼻黏膜免疫屏障功能下降,血清中多种炎症因子或炎症介质释放增加,麻黄汤和银翘散均可提高大鼠鼻黏膜免疫屏障功能,麻黄汤增加IgA含量的作用较明显,银翘散减少炎症因子的作用更明显。Objective To explore the effect of cold-warm cycle stimulation on nasal mucosal immune barrier function in rats and determine the difference between Mahuang decoction and Yinqiao powder,which are commonly used in traditional Chinese medicine.Methods For cold-warm cycle stimulation,SD rats were exposed to four cycles of-15°C for 1 hour and then 25°C for 30 minutes.At different time points after the last stimulation,the protein expression of IgA in the nasal mucosa was detected by Western blot.Seven days after the first cold-warm cycle stimulation,the rats were re-subjected to cold-warm cycle stimulation under the same conditions.The dynamic changes in IgA protein expression in the nasal mucosa,lysozyme and prostaglandin E2(PGE2)levels in nasal lavage fluid and PGE2,TNF-α,IL-6 and IL-1βserum contents were detected on days 1~11 after the last stimulation.The rats were randomly divided into negative control,model,Mahuang decoction and Yinqiao powder groups.After repeated cold-warm stimulation for 6 hours,the rats in the negative control group and model group were given distilled water,the Mahuang decoction group was given 2.43 g crude drug/kg body weight Mahuang decoction,and the Yinqiao powder group was given 16.74 g crude drug/kg body weight Yinqiao powder once a day for 7 days 30 minutes after the last stimulation.Except for the negative control group,the other groups were re-subjected to repeated cold-warm stimulation,and the medicines were given twice at 6 and 22 hours after stimulation.Nasal lavage fluid was prepared,nasal mucosal tissue was extracted,and blood was obtained for detecting the corresponding indexes at 24 hours.Results After repeated cold-warm cycle stimulation,IgA protein expression in the nasal mucosa was significantly decreased at days 1 and 3 and then gradually increased and returned to normal on day 7.When rats were re-stimulated by cold-warm cycles,IgA protein expression in the nasal mucosa was significantly decreased,and the amounts of lysozyme and PGE2 in nasal lavage fluid were signif

关 键 词：冷-热刺激 鼻黏膜免疫 炎症 解表方 

分 类 号：Q95-33[生物学—动物学]