产子囊霉素吸水链霉菌遗传操作系统的优化及调控基因的研究  

Optimization of Genetic Operating System of Ascomycin-producing Streptomyces hygroscopicus and the Study of Regulatory Genes

作　　者：刘倩倩 余志拓 吴远杰[2] 张正玉 陈少欣[2] LIU Qianqian;YU Zhituo;WU Yuanjie;ZHANG Zhengyu;CHEN Shaoxin(School of Chemistry and Chemical Engineering,Shanghai University of Engineering Science,Shanghai 201620;State Key Lab.of New Drug and Pharmaceutical Process,Shanghai Institute of Pharmaceutical Industry,China State Institute of Pharmaceutical Industry,Shanghai 201203)

机构地区：[1]上海工程技术大学化学化工学院,上海201620 [2]中国医药工业研究总院上海医药工业研究院,创新药物与制药工艺国家重点实验室,上海201203

出　　处：《中国医药工业杂志》2021年第12期1603-1608,共6页Chinese Journal of Pharmaceuticals

摘　　要：吸水链霉菌所产的子囊霉素(1)在工业上具有重要的应用价值,但存在遗传操作效率低等问题。本研究以1生产菌株吸水链霉菌SFK-6-33为研究对象,考察了影响吸水链霉菌与大肠埃希菌ET12567接合转移效率的因素,优化了吸水链霉菌与大肠埃希菌的接合转移遗传操作系统。结果表明,优化后的接合转移效率由最初的9.25×10^(–7)增加至4.33×10^(–5)。此外,在此基础上对吸水链霉菌生产1的相关调控基因(fkbN、fkbR1、shyT4和shyT10)进行研究,初步发现调控基因fkbN、shyT4能够促进1的合成,而调控基因fkbR1、shyT10对1的生物合成有抑制作用。Ascomycin(1) produced by Streptomyces hygroscopicus has important application value in industry,but it has problems such as low efficiency of genetic manipulation.Streptomyces hygroscopicus SFK-6-33 was used to investigate the factors affecting the efficiency of conjugal transfer between Streptomyces hygroscopicus and Escherichia coli ET12567,and the genetic operating system for conjugal transfer between Streptomyces hygroscopicus and Escherichia coli was optimized.The results showed that the optimized conjugation efficiency increased from the original 9.25×10^(–7) to 4.33×10^(–5).In addition,on this basis,the influences of relevant regulatory genes(fkbN,fkbR1,shyT4 and shyT10) of Streptomyces hygroscopicus on the production of 1 were investigated,and it was initially found that the regulatory genes fkbN and shyT4 could promote the synthesis of 1,while the regulatory genes fkbR1 and shyT10 were inhibitive.

关 键 词：吸水链霉菌 子囊霉素 接合转移效率 遗传操作系统 调控基因 

分 类 号：TQ465[化学工程—制药化工] R978.15[医药卫生—药品]