猪流行性腹泻与猪传染性胃肠炎二重液相芯片检测方法的建立  

Establishment of a Diplex Liquichip Method for Detection of Porcine Epidemic Diarrhea Virus and Transmissible Gastroenteritis Virus

作　　者：曹丙蕾 张群 刘敏 王群 尹伟力 Cao Binglei;Zhang Qun;Liu Min;Wang Qun;Yin Weili(Ji'nan Customs Technology Center,Ji'nan 250014,Shandong,China;Qingdao Customs Technology Center,Qingdao 266114,Shandong,China)

机构地区：[1]济南海关技术中心,山东济南250014 [2]青岛海关技术中心,山东青岛266114

出　　处：《农业技术与装备》2021年第11期144-145,147,共3页Agricultural Technology & Equipment

基　　金：畜禽疫病液相芯片高通量检测技术及东部沿海口岸监测应用示范(2016YFD050110401);原国家质检总局科研项目(2016IK193);原山东出入境检验检疫科研项目(SK201401)(SK201713);济南海关科研项目(2019JK004)。

摘　　要：为了鉴别猪流行性腹泻和猪传染性胃肠炎,研究建立了猪流行性腹泻病毒(PEDV)和猪传染性胃肠炎病毒(TGEV)的二重液相芯片检测技术。结果表明,该检测方法特异性好,可同时区分诊断PEDV和TGEV,与荧光RT-PCR检测方法的符合率为100%,可有效区分诊断2种混合感染的疾病。In order to distinguish porcine epidemic diarrhea and porcine transmissible gastroenteritis,this study established a dual liq‐uid-phase chip detection technology for porcine epidemic diarrhea virus(PEDV)and porcine transmissible gastroenteritis virus(TGEV).The results showed that the detection method had good specificity and could distinguish and diagnose PEDV and TGEV at the same time.The coincidence rate with fluorescent RT-PCR was 100%,which could effectively distinguish and diagnose two mixed infection diseases.

关 键 词：猪流行性腹泻 猪传染性胃肠炎 液相芯片 杂交 

分 类 号：S852.651[农业科学—基础兽医学]