imple Bioassay for PAMP-Triggered Immunity in Rice Seedlings Based on Lateral Root Growth Inhibition  

作　　者：WANG Rui ZHANG Dandan LI Shengnan GAO Jinlan HAN Liebao QIU Jinlong 

机构地区：[1]College of Grassland Science,Beijing Forestry University,Beijing 100083,China [2]State Key Laboratory of Plant Genomics,Institute of Microbiology,Chinese Academy of Sciences(CAS),Beijing 100101,China [3]CAS Center for Excellence in Biotic Interactions,University of Chinese Academy of Sciences,Beijing 100049,China

出　　处：《Rice science》2022年第1期67-75,I0025,共10页水稻科学（英文版）

基　　金：supported by the National Key Research and Development Program of China(Grant No.2016YFD0100602);the National Natural Science Foundation of China(Grant No.31901868)。

摘　　要：Pathogen-associated molecular pattern(PAMP)-triggered immunity(PTI)is an essential layer of plant disease resistance.Robust bioassays for PTI are pre-required to dissect its molecular mechanism.In this study,we established that lateral root growth inhibition as a simple and robust measurement of PTI in rice seedlings.Specifically,flg22,a well-characterized PAMP from bacterial flagellin,was used to induce PTI in rice seedlings.While flg22 treatment induced PR gene expression and mitogen-activated protein kinase activation in the roots of rice seedlings to support the PTI triggered,this treatment substantially repressed lateral root growth,but it did not alter primary root growth.Moreover,treatments with chitin(i.e.,a fungal PAMP)and oligogalacturonides(i.e.,classical damage-associated molecular pattern)clearly inhibited the lateral root growth,although a priming step involving ulvan was required for the chitin treatment.The bioassay developed was applicable to various rice cultivars and wild species.Thus,lateral root growth inhibition represents a simple and reliable assay for studying PTI in rice plants.

关 键 词：pathogen-associated molecular pattern(PAMP) PAMP-triggered immunity lateral root growth mitogen-activated protein kinase RICE flg22 

分 类 号：S511[农业科学—作物学]