基于两种蛋白质组学技术筛选不同分期白癜风的差异表达蛋白  

Proteomics study of differentially expressed proteins in patients with different stages of vitiligo

作　　者：李一雷 高兴华[2] 杨荞榕 徐欣植 杨骥 李明 LI Yi-lei;GAO Xing-hua;YANG Qiao-rong;XU Xin-zhi;YANG Ji;LI Ming(Department of Dermatology,Zhongshan Hospital,Fudan University,Shanghai 200032,China;Department of Dermatology,the First Hospital of China Medical University,Shenyang 110001,Liaoning,China)

机构地区：[1]复旦大学附属中山医院皮肤科,上海200032 [2]中国医科大学附属第一医院皮肤科,沈阳110001

出　　处：《中国临床医学》2021年第6期925-933,共9页Chinese Journal of Clinical Medicine

基　　金：国家自然科学基金青年科学基金(82003345).

摘　　要：目的:采用两种蛋白质组学技术鉴定和筛选不同分期白癜风的血清标志物,并研究其网络关系。方法:收集白癜风稳定期和进展期以及健康人各15例的血清样本,采用双向凝胶电泳技术(2-DE)和核素标记相对和绝对定量技术(iTRAQ)对所有样本进行检测,筛选出差异表达蛋白,并用软件分析其可能作用的主要通路。结果:通过2-DE鉴定出稳定期白癜风患者10个差异蛋白(6个上调,4个下调),进展期白癜风患者25个差异蛋白(11个上调,14个下调);iTRAQ鉴定出稳定期患者62个差异蛋白(29个上调,33个下调),进展期患者50个差异蛋白(30个上调,20个下调)。两种蛋白质组学鉴定出的相同差异蛋白包括稳定期间α-胰蛋白酶抑制剂重链H4、补体C4-A(上调);进展期补体C4-B、载脂蛋白A(下调)和间α-胰蛋白酶抑制剂重链H4(上调)。基因本体(Gene ontology,GO)注释分析示共同的差异蛋白主要参与CCKR通路、纤溶酶原激活级联通路、p53通路、趋化炎症因子调控通路等。结论:基于2-DE与iTRAQ两种不同的蛋白质组学方法筛选出不同分期白癜风的共同差异表达蛋白,为进一步研究白癜风的发生机制奠定了基础。Objective:To identify and screen serum markers in different stages of vitiligo by 2 proteomic techniques,and to explore their relationship network.Methods:Serum samples were collected from 15 patients with vitiligo in stable stage,15 patients with vitiligo in progressive stage,and 15 healthy individuals.Two-dimensional gel electrophoresis(2-DE)and isobaric tags for relative and absolute quantification(iTRAQ)were used to detect and label all samples.Differential proteins were screened,and the relationship of pathways was analyzed by software.Results:A total of 10 differential proteins were identified by 2-DE in patients with stable vitiligo,including 6 up-regulated and 4 down-regulated proteins;25 differential proteins were identified in patients with progressive vitiligo,including 11 up-regulated and 14 down-regulated proteins.Six-two differential proteins(29 up-regulated and 33 down-regulated)in stable patients,and 50 differential proteins(30 up-regulated and 20 down-regulated)in advanced patients were identified by iTRAQ.The same differential proteins identified by the two proteomics includeα-trypsin inhibitor heavy chain H4,complement C4-A(up-regulated)at the stable stage of vitiligo,and complement C4-B,apolipoprotein A(down-regulated)andα-trypsin inhibitor heavy chain H4(up-regulated)at the advanced stage of vitiligo.According to GO annotation,the main pathways involved in the differential proteins were CCKR,plasminogen activator,p53,chemokine regulatory pathways,etc.Conclusions:The differentially expressed proteins in different stages of vitiligo could be screened by proteomic methods of both 2-DE and iTRAQ technology,which provided a foundation for further study of the pathogenesis of vitiligo.

关 键 词：双向凝胶电泳技术 核素标记相对和绝对定量技术 白癜风 分期 差异蛋白 

分 类 号：R758.41[医药卫生—皮肤病学与性病学]