非洲辣木种子无菌萌发与组培再生体系的建立  

Establishment of Aseptic Germination and Tissue Culture Regeneration System of Moringa stenopetala Seeds

作　　者：覃玉凤[1] 钟连香 魏秋兰 肖玉菲 覃子海[1] QIN Yufeng;ZHONG Lianxiang;WEI Qiulan;XIAO Yufei;QIN Zihai(Guangxi Academy of Forestry, Nanning 530002, China)

机构地区：[1]广西壮族自治区林业科学研究院,广西南宁530002

出　　处：《林业调查规划》2021年第6期18-22,共5页Forest Inventory and Planning

基　　金：广西科技计划项目(桂科AD18281083)资助.

摘　　要：以非洲辣木种子为材料,探究非洲辣木种子的最佳消毒方法,同时研究培养基和植物生长调节剂对非洲辣木无菌苗诱导、继代增殖及生根的影响,建立组培再生体系。结果表明,非洲辣木种子处理和消毒的最佳方式为剥壳+清水0.5 h+75%酒精60 s+0.1%升汞10 min,此处理的非洲辣木种子发芽率高达81.11%,而污染率仅为17.41%。最佳的初代诱导培养基为MS+6-BA 1.0 mg/L+KT 0.4 mg/L,每个外植体平均萌芽数为3.17;初代芽在MS+6-BA 0.6 mg/L+KT 0.3 mg/L的培养基上进行继代增殖培养,增殖系数可达到4.62;在1/2 MS+NAA 1.0 mg/L+IBA 0.5 mg/L的培养基上进行生根培养,株均根数达5.88条,平均根长在2.0 cm以上,平均生根率达86.68%。The best disinfection method of Moringa stenopetala seeds was explored,and the effects of medium and plant growth regulator on induction,subculture multiplication and rooting of aseptic seedlings of Moringa stenopetala were studied.The results showed that the best disinfection method was shelling+water for 0.5 h+75%alcohol for 60 s+0.1%mercuric chloride for 10 min.The germination rate of sterilized seeds was as high as 81.11%,while the pollution rate was only 17.41%.The best medium for primary induction was MS+6-BA 1.0 mg/L+KT 0.4 mg/L,and the average number of buds per explant was 3.17.The primary buds were subcultured on the medium of MS+6-BA 0.6 mg/L+KT 0.3 mg/L,and the proliferation coefficient was 4.62.The average number of roots was 5.88,the average root length was more than 2.0 cm,and the average rooting rate reached 86.68%on the medium of 1/2 MS+NAA 1.0 mg/L+IBA 0.5 mg/L.

关 键 词：非洲辣木 组织培养 消毒方法 再生体系 无菌苗诱导 继代增殖 

分 类 号：S723.133[农业科学—林木遗传育种] Q949.748.5[农业科学—林学]