Elucidation of the life cycle of the endophytic genus Muscodor and its transfer to Induratia in Induratiaceae fam. nov., based on a polyphasic taxonomic approach  

作　　者：Milan C.Samarakoon Benjarong Thongbai Kevin D.Hyde Mark Brönstrup Ulrike Beutling Christopher Lambert Andrew N.Miller Jian‑Kui(Jack)Liu Itthayakorn Promputtha Marc Stadler 

机构地区：[1]Key Laboratory for Plant Diversity and Biogeography of East Asia,Kunming Institute of Botany,Chinese Academy of Sciences,132,Lanhei Road,Kunming 650201,People’s Republic of China [2]Center of Excellence in Fungal Research,Mae Fah Luang University,Chiang Rai 57100,Thailand [3]School of Life Science and Technology,University of Electronic Science and Technology of China,Chengdu 611731,People’s Republic of China [4]Guizhou Key Laboratory of Agricultural Biotechnology,Guizhou Academy of Agricultural Sciences,Guiyang 550006,People’s Republic of China [5]Department of Microbial Drugs,Helmholtz-Zentrum für Infektionsforschung GmbH,Inhoffenstrasse 7,38124 Brunswick,Germany [6]Department of Biology,Faculty of Science,Chiang Mai University,Chiang Mai 50200,Thailand [7]Department of Chemical Biology,Helmholtz-Zentrum für Infektionsforschung GmbH,Inhoffenstrasse 7,38124 Brunswick,Germany [8]Illinois Natural History Survey,University of Illinois,1816 South Oak Street,Champaign,IL 61820‑6970,USA [9]Research Center in Bioresources for Agriculture,Industry and Medicine,Chiang Mai University,Chiang Mai 50200,Thailand

出　　处：《Fungal Diversity》2020年第2期177-210,共34页真菌多样性（英文）

基　　金：Kevin D.Hyde would like to thank the Thailand Research Fund for a grant entitled“Impact of climate change on fungal diversity and biogeography in the Greater Mekong Sub region(grant no:RDG6130001)for supporting this study.Jian-Kui(Jack)Liu thanks the Joint Fund of the National Natural Science Foundation of China and the Karst Science Research Center of Guizhou province(Grant No.U1812401);This research work was supported by Chiang Mai University.Milan C.Samarakoon is grateful to the Mushroom Research Foundation(MRF),Chiang Rai,Thailand for providing a Postgraduate Scholarship.Mark Brönstrup thanks for support from EMBRIC,the EU-funded European Marine Biological Research Infrastructure Cluster[654008].

摘　　要：Molecular phylogenetic studies of cultures derived from some specimens of plant-inhabiting Sordariomycetes using ITS,LSU,rpb2 and tub2 DNA sequence data revealed close affinities to strains of Muscodor.The taxonomy of this biotechnologi-cally important genus,which exclusively consists of endophytes with sterile mycelia that produce antibiotic volatile secondary metabolites,was based on a rather tentative taxonomic concept.Even though it was accommodated in Xylariaceae,its phylo-genetic position had so far remained obscure.Our phylogeny shows that Muscodor species have affinities to the xylarialean genera Emarcea and Induratia,which is corroborated by the fact that their sexual states produce characteristic apiospores.These data allow for the integration of Muscodor in Induratia,i.e.the genus that was historically described first.The multi-locus phylogenetic tree clearly revealed that a clade comprising Emarcea and Induratia forms a monophylum separate from representatives of Xylariaceae,for which we propose the new family Induratiaceae.Divergence time estimations revealed that Induratiaceae has been diverged from the Xylariaceae+Clypeosphaeriaceae clade at 93(69-119)million years ago(Mya)with the crown age of 61(39-85)Mya during the Cretaceous period.The ascospore-derived cultures were studied for the production of volatile metabolites,using both,dual cultures for assessment of antimicrobial effects and extensive analyses using gas chromatography coupled with mass spectrometry(GC-MS).The antimicrobial effects observed were significant,but not as strong as in the case of the previous reports on Muscodor species.The GC-MS results give rise to some doubt on the validity of the previous identification of certain volatiles.Many peaks in the GC-MS chromatograms could not be safely identified by database searches and may represent new natural products.The isolation of these compounds by preparative chromatography and their subsequent characterisation by nuclear magnetic resonance(NMR)spectroscopy or total synthesis will

关 键 词：9 new taxa 16 new combinations Biocontrol ENDOPHYTES Secondary metabolites SORDARIOMYCETES 

分 类 号：O62[理学—有机化学]