AM真菌对留兰香和常夏石竹耐盐性的影响  

Effect of AM Fungi on the Salt Tolerance of Mentha spicata and Dianthus plumarius

作　　者：徐嘉美 郭静怡 吴杨 杨春雪[1] XU Jiamei;GUO Jingyi;WU Yang;YANG Chunxue(College of Landscape Architecture, Northeast Forestry University, Harbin 150000, China)

机构地区：[1]东北林业大学园林学院,哈尔滨150000

出　　处：《西北植物学报》2021年第12期2104-2112,共9页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金青年科学基金(31601986);黑龙江省博士后科研启动金(LBH-Q16005)。

摘　　要：为探究接种丛枝菌根(arbuscular mycorrhiza,AM)真菌对不同盐胁迫水平下留兰香和常夏石竹侵染特性与生理指标的影响,该研究采用盆栽试验的方法,将留兰香和常夏石竹分为接种处理与对照处理,并施加不同浓度(0、50、100、150、200 mmol/L)的NaCl胁迫,胁迫结束后测定两种植物的侵染特性与生理指标。结果表明:(1)随着盐浓度的升高,留兰香和常夏石竹的侵染率、侵染强度、丛枝丰度和泡囊丰度均不断下降,且常夏石竹的各项侵染指标总体上均高于留兰香。(2)接种AM真菌提高了各盐浓度下留兰香和常夏石竹的总叶绿素含量以及可溶性糖与可溶性蛋白含量,同时显著降低了二者在不同盐浓度条件下的脯氨酸含量。(3)接种AM真菌在不同程度上提高了留兰香和常夏石竹体内超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)和抗坏血酸过氧化物酶(APX)的活性;并降低了在不同盐浓度条件下留兰香和常夏石竹的丙二醛含量。研究发现,接种AM真菌可以在不同程度上提高盐胁迫下留兰香和常夏石竹渗透调节能力和抗氧化酶系统活性,增强了植物的耐盐能力,从而使植物在盐胁迫条件下更好地生长。In order to explore the effects of inoculation of arbuscular mycorrhiza(AM)fungi on the colonization characteristics and physiological indexes of Mentha spicata and Dianthus plumarius under different salt stress levels,we used the method of pot experiment in this study to divide M.spicata and D.plumarius into inoculation treatment and control treatment,and applied different concentrations(0,50,100,150,200 mmol/L)of NaCl stress.After the end of the stress,we measured the colonization characteristics and physiological indicators of the two plants.The results showed that:(1)with the increase of salt concentration,the colonization rate,colonization intensity,arbuscular abundance and vesicle abundance of M.spicata and D.plumarius continued to decrease,and the various colonization indexes of D.plumarius are generally higher than that of M.spicata.(2)Inoculation of AM fungi increased the total chlorophyll content,soluble sugar and soluble protein contents of M.spicata and D.plumarius in each salt concentration,while significantly reducing the proline content of the two under different salt concentrations.(3)Inoculation with AM fungi increased the activities of superoxide dismutase(SOD),peroxidase(POD),catalase(CAT)and ascorbate peroxidase(APX)in M.spicata and D.plumarius to varying degrees,and reduced the malondialdehyde content of M.spicata and D.plumarius under different salt concentration.The study found that inoculation with AM fungi can improve the osmotic adjustment ability and antioxidant enzyme system activity of M.spicata and D.plumarius in different degrees under salt stress,enhance the salt tolerance of plants,and make plants grow better under salt stress conditions.

关 键 词：留兰香 常夏石竹 盐胁迫 丛枝菌根真菌 耐盐性 

分 类 号：Q945.78[生物学—植物学]