Effects of scandium chloride on osteogenic and adipogenic differentiation of mesenchymal stem cells  

作　　者：Na Ren Xin Yu Aizhu Wang Na Liang Zhichao Feng Chunhui Sun 

机构地区：[1]Collaborative Innovation Center of Technology and Equipment for Biological Diagnosis and Therapy in Universities of Shandong,Institute for Advanced Interdisciplinary Research(iAIR),University of Jinan,Jinan 250022,China [2]School of Biological Science and Technology,University of Jinan,Jinan 250022,China

出　　处：《Journal of Rare Earths》2022年第1期161-168,共8页稀土学报（英文版）

基　　金：Project supported by the National Natural Science Foundation of China(51972148,51802115,11904131,21501090);the Project of"20 items of University"of Jinan(2018GXRC031);the Doctoral Fund of University of Jinan(XBS1609)。

摘　　要：Mesenchymal stem cells(MSCs)are multi-potent cells that are able to differentiate and mature into various types of cells under a certain microenvironment for cell therapy and tissue regeneration.Scandium(Sc),an important rare earth element,recently has been intensively investigated in biomedical fields as well as industrial engineering,and chloride channels have been proven to be able to affect osteogenic differentiation.Thus,it is significant to investigate effects of ScCl_(3)on cell activities of MSCs.In this paper,rat bone MSCs(rBMSCs)were co-cultured with various concentrations of ScCl_(3)(1×10^(-8),1×10^(-6),and 1×10^(-4)mol/L)to evaluate their influence on cell proliferation as well as osteogenic and adipogenic differentiation in vitro.The results indicate that ScCl_(3)promotes the proliferation of rBMSCs initially,which is yet reduced upon ion accumulation.We used immunofluorescence staining,quantitative real time polymerase chain reactions,and assays measuring alkaline phosphatase activity,mineralized deposits,and intracytoplasmic lipids to reveal that rBMSCs treated with ScCl_(3)at concentrations of 1×10^(-8)-1×10^(-6)mol/L can enhance levels of osteogenic differentiation in a dosedependent manner and reduce adipogenic differentiation to a certain degree through Wnt/β-catenin signaling pathway.These results indicate that appropriate concentrations of ScCl_(3)can improve osteogenic differentiation in the lineage commitment of rBMSCs,and thus,promote bone remodeling.This study implies that ScCl_(3) possesses great potentials in the treatment of bone diseases and would provide new strategy of designing composites by SiCl3 doping for biomedical applications in the future.

关 键 词：SCANDIUM Mesenchymal stem cells Osteogenic differentiation Adipogenic differentiation Dose-dependent effects Wnt/3-catenin signaling pathway 

分 类 号：R318.08[医药卫生—生物医学工程] TQ133.3[医药卫生—基础医学]