Genome-wide analysis of the CCCH zinc finger family in longan:Characteristic identification and expression profiles in Dimocarpus longan Lour  

作　　者：SU Li-yao XIAO Xue-chen JIANG Meng-qi HUANG Shu-qi XUE Xiao-dong LI Xue LAI Zhong-xiong LIN Yu-ling 

机构地区：[1]Institute of Horticultural Biotechnology,Fujian Agriculture and Forestry University,Fuzhou 350002,P.R.China

出　　处：《Journal of Integrative Agriculture》2022年第1期113-130,共18页农业科学学报（英文版）

基　　金：funded by the National Natural Science Foundation of China(31672127 and 31572088);the Scientific Research Foundation of Horticulture College of Fujian Agriculture and Forestry University,China(2018S02);the Construction of Plateau Discipline of Fujian Province,China(102/71201801101);the Project of Fujian Academy of Agricultural Sciences,China(AB2017-4)。

摘　　要：CCCH(C3 H) Zinc finger(Znf) transcription factors(TFs), as a novel type of Znf gene, regulate the expression of genes by binding to their mRNAs and play important roles in plant growth and development and abiotic stress resistance.Longan(Dimocarpous longan) is a tropical/subtropical fruit tree of great economic importance in Southeast Asia.However, genomic information on C3 H and their functions in longan are still unknown. In this study, a comprehensive analysis of the longan C3 H(DlC3 H) gene family was carried out. A total of 49 DlC3 H genes in three clades were identified from the longan genome database. Characteristics of the genes were analyzed with respect to gene structure,motif composition, phylogenetic tree and potential functions. The analysis of alternative splicing(AS) events suggested that AS events in DlC3 H genes were related to the transformation from longan non-embryonic to embryonic cultures.Promoter analysis indicated that most of the DlC3 H genes included cis-acting elements associated with hormones and stresses responses. Quantitative real-time PCR(qRT-PCR) analysis indicated that 26 of the 49 DlC3 Hs, which possess methyl jasmonate(MeJA) and abscisic acid(ABA) responsive cis-acting elements, showed differential expression patterns under treatment with ABA, MeJA and their endogenous inhibitors, suggesting that DlC3 Hs might be involved in the ABA and MeJA signaling pathways. The expression profiles of 17 of the 49 DlC3 Hs in non-embryonic callus and three tissues of embryonic cultures showed that only five of the 17 DlC3 Hs had the same expression trends as the FPKM trends in transcriptome data;the expression levels of DlC3 H07/14/16/36/49 in embryogenic callus and DlC3 H04/38 in globular embryos were high, suggesting that they have different functions in embryonic development. Further, we verified that DlC3 H01/03/05/11/19/39 were regulated by sRNAs by a modified 5’ RLM-RACE method. This study provides the first systematic analysis of C3 H genes in longan, and found that C3 H genes may be

关 键 词：Dimocarpus longan Lour CCCH Zinc finger characteristic identification expression profile 

分 类 号：S667.2[农业科学—果树学]