Constructing the wolfberry(Lycium spp.) genetic linkage map using AFLP and SSR markers  

作　　者：YIN Yue AN Wei ZHAO Jian-hua LI Yan-long FAN Yun-fang CHEN Jin-huan CAO You-long ZHAN Xiang-qiang 

机构地区：[1]State Key Laboratory of Crop Stress Biology for Arid Areas,College of Horticulture,Northwest A&F University,Yangling 712100,P.R.China [2]National Wolfberry Engineering Research Center,Ningxia Academy of Agricultural and Forestry Sciences,Yinchuan 751002,P.R.China [3]College of Biological Sciences and Technology,Beijing Forestry University,Beijing 100083,P.R.China

出　　处：《Journal of Integrative Agriculture》2022年第1期131-138,共8页农业科学学报（英文版）

基　　金：financially supported by the National Natural Science Foundation of China(31760218);the Third Batch of Ningxia Youth Talents Supporting Program(TJGC2018022);the Whole Industry Chain Innovation Demonstration Project of Ningxia Academy of Agriculture and Forestry Sciences,China(ERS-2016-0405)。

摘　　要：Genetic linkage maps are important for quantitative trait locus(QTL) and marker-assisted selection breeding. The wolfberry(Lycium spp.) is an important food and traditional medicine in China. However, few construction genetic linkage maps have been reported because of the lack of genomic and genetic resources. In this study, a population of 89 F1 seedings was derived from a cross between two heterozygous parents, L. chinense var. potaninii ‘BF-01’(female)and L. barbarum var. auranticarpum ‘NH-01’(male), in order to construct a genetic linkage map using simple sequence repeat(SSR) and amplified fragment length polymorphism(AFLP) markers based on the double pseudo-test cross mapping strategy. The resulting genetic map consisted of 165 markers(74 AFLPs and 91 SSRs) distributed across 12 linkage groups and spanned a total length of 557.6 cM with an average distance of 3.38 cM between adjacent markers.The 12 linkage groups contained 3 to 21 markers and ranged in length from 8.6 to 58.3 cM. Twenty-nine segregated markers distributed in the map were mainly located on LG4 and LG9 linkage groups at P<0.05. This is the first linkage map of Lycium species using SSR and AFLP markers, which can serve as basis for improving genes and selective breeding of the genome assembly.

关 键 词：LYCIUM genetic linkage map AFLP SSR 

分 类 号：S567.19[农业科学—中草药栽培]