Characterization and fine mapping of RTMS10, a semi-dominant reverse thermo-sensitive genic male sterile locus in rice  

作　　者：NI Jin-long WANG De-zheng NI Da-hu SONG Feng-shun YANG Jian-bo YAO Da-nian 

机构地区：[1]College of Agronomy,Anhui Agricultural University,Hefei 230036,P.R.China [2]Rice Genetics and Breeding Key Laboratory of Anhui Province/Rice Research Institute,Anhui Academy of Agricultural Sciences,Hefei 230031,P.R.China

出　　处：《Journal of Integrative Agriculture》2022年第2期316-325,共10页农业科学学报（英文版）

基　　金：funded by the Key Research and Development Program of Anhui Province,China(201904a06020016 and 202104g01020013);the National Natural Science Foundation of China(31101204);the Program of Rice Genetic Breeding Key Laboratory of Anhui Province,China(SDKF-201903)。

摘　　要：The discovery and application of environment-sensitive genic male sterile(EGMS) rice germplasm provide an easy method for hybrid rice breeding and have made great contributions to hybrid rice production. Typically, the photoperiod-and thermosensitive GMS(P/TGMS) lines utilized in two-line hybrid systems are male sterile under long day or/and high temperature but fertile under short day or/and low temperature conditions. However, Yannong S(Yn S), a reverse TGMS(rTGMS) line, is sterile under low temperature(<29℃) and fertile under high temperature(>29.5℃). Here, we report a genetic study on the rTGMS trait in Yn S. Interestingly, the F1 plants of the cross between Yn S and a cultivar, L422, were male sterile at 22℃ and completely fertile at 27℃. Moreover, the segregation ratio of fertile and sterile individuals in Yn S/L422 F2 populations changed from 1:3.05 to 2.95:1 when the ambient temperature increased, showing that the rTGMS trait exhibits semidominance in Yn S. We further found a locus on chromosome 10, termed RTMS10, which controls the rTGMS trait in Yn S. We then finely mapped RTMS10 to a ~68 kb interval between markers ID13116 and ID1318 by Yn S/L422 BC6 F2 populations. A near iso-genic line(NIL) NL1 from the BC6 F3 generation was developed and the pollen of NL1 became abnormal from the meiosis stage under low temperature. In summary, we identified an rTGMS locus, RTMS10, and provided co-segregated markers, which could help to accelerate molecular breeding of rTGMS lines and better understand the rTGMS trait in rice.

关 键 词：RICE photoperiod-and thermo-sensitive genic male sterile(P/TGMS) reverse TGMS(rTGMS) gene mapping RTMS10 

分 类 号：S511[农业科学—作物学]